Medial auditory thalamic input to the lateral pontine nuclei is necessary for auditory eyeblink conditioning.
Medial auditory thalamic input to the lateral pontine nuclei is necessary for auditory eyeblink conditioning.
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DOI：
10.1016/j.nlm.2009.08.008
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发表时间：
2010-01
影响因子：
2.7
通讯作者：
Freeman, John H.
中科院分区：
文献类型：
--
作者：
Halverson, Hunter E.;Freeman, John H.
通过可逆性失活脑桥内侧核(MPN)或外侧核(LPN)，研究了眨眼条件反射的听觉和视觉条件刺激(CS)通路。在实验1中，Long-Evans大鼠被给予三个阶段的眨眼条件反射。第一阶段包括三次训练，电刺激丘脑内侧听神经核团(MATN)，配合眼眶周围电击无条件刺激(US)。另一次治疗是在不输液的情况下，以蝇香酚(L 0.5微米，10 mm)或生理盐水注射为靶点，然后进行恢复治疗。然后，在阶段2和阶段3(平衡)中用音调或轻音CS重复相同的训练和测试序列。实验2包括与实验1相同的训练，只是在保持测试期间向MPN中注入蝇草酚或生理盐水。以LPN为靶点的Muscimol注射严重损害了对MATN刺激和纯音CS的眨眼条件反应(CRS)的保持，但仅部分减少了对光CS的CR百分比。与MATN刺激或纯音CS相比，以MPN为靶点的Muscimol对轻CS的CR保留的影响更大。结果表明，延迟瞬目条件反射所需的听觉CS通路包括MATN-LPN投射，视觉CS通路包括MPN。
Auditory and visual conditioned stimulus (CS) pathways for eyeblink conditioning were investigated with reversible inactivation of the medial (MPN) or lateral (LPN) pontine nuclei. In Experiment 1, Long-Evans rats were given three phases of eyeblink conditioning. Phase 1 consisted of three training sessions with electrical stimulation of the medial auditory thalamic nuclei (MATN) paired with a periorbital shock unconditioned stimulus (US). An additional session was given with a muscimol (0.5 µL, 10 mM) or saline infusion targeting the LPN followed by a recovery session with no infusions. The same training and testing sequence was then repeated with either a tone or light CS in phases 2 and 3 (counterbalanced). Experiment 2 consisted of the same training as Experiment 1 except that muscimol or saline was infused in the MPN during the retention tests. Muscimol infusions targeting the LPN severely impaired retention of eyeblink conditioned responses (CRs) to the MATN stimulation and tone CSs but only partially reduced CR percentage to the light CS. Muscimol infusions that targeted the MPN had a larger effect on CR retention to the light CS relative to MATN stimulation or tone CSs. The results provide evidence that the auditory CS pathway necessary for delay eyeblink conditioning includes the MATN-LPN projection and the visual CS pathway includes the MPN.
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影响因子：
2
作者：
Freeman, JH;Rabinak, CA;Campolattaro, MM
通讯作者：
Campolattaro, MM
影响因子：
56.9
作者：
KRUPA, DJ;THOMPSON, JK;THOMPSON, RF
通讯作者：
THOMPSON, RF
DOI：
10.1101/lm.1002508
发表时间：
2008-07
期刊：
Learning & memory (Cold Spring Harbor, N.Y.)
影响因子：
--
作者：
Halverson HE;Poremba A;Freeman JH
通讯作者：
Freeman JH
影响因子：
16.2
作者：
Hesslow, G;Svensson, P;Ivarsson, M
通讯作者：
Ivarsson, M
影响因子：
1.9
作者：
Garcia, KS;Mauk, MD;Kehoe, EJ
通讯作者：
Kehoe, EJ