Medial auditory thalamus inactivation prevents acquisition and retention of eyeblink conditioning.
Medial auditory thalamus inactivation prevents acquisition and retention of eyeblink conditioning.
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DOI：
10.1101/lm.1002508
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发表时间：
2008-07
期刊：
影响因子：
--
通讯作者：
Freeman JH
中科院分区：
文献类型：
--
作者：
Halverson HE;Poremba A;Freeman JH
本研究采用可逆性失活丘脑内侧听觉核团（MATN）的方法，探讨了延迟眨眼条件反射所必需的听觉条件刺激（CS）通路。在使用听觉CS的四次条件训练中的每一次之前，将盐水或蝇蕈醇输注到训练眼的对侧MATN中。然后给大鼠额外四次没有输注的训练，以评估初始训练的节省。然后对所有大鼠进行蝇蕈醇输注的保留试验，然后进行恢复期。通过MGm 0.5 mm范围内的插管输注蝇蕈醇可防止眨眼条件反应（CR）的获得，并阻止CR保留。距离MATN超过0.5 mm的套管放置不会完全阻断CR采集，并且对CR保留有部分影响。用2-脱氧葡萄糖（2-DG）放射自显影检查MATN灭活的主要和次要影响。听觉丘脑中2-DG摄取的差异与插管放置和行为结果一致。在同侧听觉皮层、脑桥基底核和下丘中发现组间2-DG摄取的差异。从这个实验的结果表明，MATN对侧的训练眼和它的投射到脑桥核是必要的收购和保留眨眼CR的听觉CS。
The auditory conditioned stimulus (CS) pathway that is necessary for delay eyeblink conditioning was investigated using reversible inactivation of the medial auditory thalamic nuclei (MATN) consisting of the medial division of the medial geniculate (MGm), suprageniculate (SG), and posterior intralaminar nucleus (PIN). Rats were given saline or muscimol infusions into the MATN contralateral to the trained eye before each of four conditioning sessions with an auditory CS. Rats were then given four additional sessions without infusions to assess savings from the initial training. All rats were then given a retention test with a muscimol infusion followed by a recovery session. Muscimol infusions through cannula placements within 0.5 mm of the MGm prevented acquisition of eyeblink conditioned responses (CRs) and also blocked CR retention. Cannula placements more than 0.5 mm from the MATN did not completely block CR acquisition and had a partial effect on CR retention. The primary and secondary effects of MATN inactivation were examined with 2-deoxy-glucose (2-DG) autoradiography. Differences in 2-DG uptake in the auditory thalamus were consistent with the cannula placements and behavioral results. Differences in 2-DG uptake were found between groups in the ipsilateral auditory cortex, basilar pontine nuclei, and inferior colliculus. Results from this experiment indicate that the MATN contralateral to the trained eye and its projection to the pontine nuclei are necessary for acquisition and retention of eyeblink CRs to an auditory CS.
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影响因子：
2
作者：
Freeman, JH;Rabinak, CA;Campolattaro, MM
通讯作者：
Campolattaro, MM
影响因子：
2
作者：
Freeman, John H.;Halverson, Hunter E.;Hubbard, Erin M.
通讯作者：
Hubbard, Erin M.
影响因子：
1.9
作者：
EDELINE, JM;WEINBERGER, NM
通讯作者：
WEINBERGER, NM
影响因子：
16.2
作者：
Hesslow, G;Svensson, P;Ivarsson, M
通讯作者：
Ivarsson, M
影响因子：
2.4
作者：
KETTNER, RE;THOMPSON, RF
通讯作者：
THOMPSON, RF