Comparison of Antifungal Activity of Bacillus Strains against Fusarium graminearum In Vitro and In Planta.
Comparison of Antifungal Activity of Bacillus Strains against Fusarium graminearum In Vitro and In Planta.
复制标题
DOI：
10.3390/plants11151999
复制
发表时间：
2022-07-31
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
小麦赤霉病（Fusarium graminearum, Fg）引起小麦和大麦赤霉病（Fusarium head blight， FHB）。这种病原体产生霉菌毒素，包括脱氧雪腐镰刀菌醇（DON）、T-2和伏莫菌素B1。谷物中真菌毒素的易位造成产量的重大损失，并对人类和牲畜造成严重的健康问题。我们在离体和植物中测试了芽孢杆菌菌株QST713 （Serenade®）和FZB24 （TAEGRO®）两种商品菌株和一种非商品菌株EU07作为微生物生物防治剂对禾本科禾谷假单胞菌Fg-K1-4的作用。EU07菌株对Fg-K1-4的抑制效果较好。无细胞细菌培养对Fg-K1-4表现出明显的拮抗作用。值得注意的是，加热和蛋白酶K处理没有降低芽孢杆菌培养物的拮抗活性。DON试验表明，培养基中DON的存在对芽孢杆菌菌株没有影响。用短柄草（Brachypodium distachyon, Bd-21）进行的叶片和头部感染实验表明，EU07在体内抑制Fg-K1-4的生长，促进植物生长。总体而言，EU07菌株表现较好，表明该菌株可用于谷物作物FHB疾病的分子研究和保护。
Fusarium graminearum (Fg) causes Fusarium head blight (FHB) disease in wheat and barley. This pathogen produces mycotoxins including deoxynivalenol (DON), the T-2 and fumorisin B1. Translocation of the mycotoxins in grains causes important losses in yields and contributes to serious health problems in humans and livestock. We tested the Bacillus strains, two commercial, QST713 (Serenade®) and FZB24 (TAEGRO®) and one non-commercial strain EU07 as microbial biological control agents against the F. graminearum strain Fg-K1-4 both in vitro and in planta. The EU07 strain showed better performance in suppressing the growth of Fg-K1-4. Cell-free bacterial cultures displayed significant antagonistic activity on Fg-K1-4. Remarkably, heat and proteinase K treatment of bacterial broths did not reduce the antagonistic activity of Bacillus cultures. DON assays showed that Bacillus strain was not affected by the presence of DON in the media. Leaf and head infection assays using Brachypodium distachyon (Bd-21) indicated that EU07 inhibits Fg-K1-4 growth in vivo and promotes plant growth. Overall, the EU07 strain performed better, indicating that it could be explored for the molecular investigations and protection of cereal crops against FHB disease.
登录
查看更多内容
影响因子：
5.2
作者：
Fan B;Wang C;Song X;Ding X;Wu L;Wu H;Gao X;Borriss R
通讯作者：
Borriss R
影响因子：
4.2
作者：
Hu, Han Qiao;Li, Xin Shen;He, Hong
通讯作者：
He, Hong
影响因子：
2.7
作者：
Beccari, G.;Prodi, A.;Covarelli, L.
通讯作者：
Covarelli, L.
DOI：
10.1080/19440040903571747
发表时间：
2010-01-01
影响因子：
2.9
作者：
Awad, Wageha A.;Ghareeb, Khaled;Zentek, Juergen
通讯作者：
Zentek, Juergen
影响因子：
2.8
作者：
Dweba, C. C.;Figlan, S.;Tsilo, T. J.
通讯作者：
Tsilo, T. J.