Transcriptional analysis of late ripening stages of grapevine berry.
Transcriptional analysis of late ripening stages of grapevine berry.
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DOI：
10.1186/1471-2229-11-165
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发表时间：
2011-11-18
影响因子：
5.3
通讯作者：
Delrot S
中科院分区：
文献类型：
--
作者：
Guillaumie S;Fouquet R;Kappel C;Camps C;Terrier N;Moncomble D;Dunlevy JD;Davies C;Boss PK;Delrot S
葡萄浆果在收获时的成分是葡萄酒质量的主要决定因素。浆果的最佳酿酒成熟度特点是糖酸比高，果皮花青素含量高，收敛程度低。然而，采摘时间仍然主要是基于粗制的生化成分和浆果品尝来确定的。在此背景下，确定哪些基因在成熟后期特异表达/抑制，并可用作成熟度的指标是很有趣的。连续两年在霞多丽葡萄(白葡萄品种)上采集整串和浆果，分三个成熟阶段(采收前7天(TH-7)、采收(TH)和采收后10天(TH+10))。显微酿造和感官分析表明，在TH-7、TH和TH+10收集的整串葡萄酒的质量不同，从而提供了最高质量的葡萄酒。同时，用Qiagen/Operon微阵列研究了两种类型的样品，即整束样品和按密度分类的浆果样品。在霞多丽研究的两年中，只有12个基因在整串和密度分选的浆果中持续上调或下调。有52个基因在TH-7和TH样本中有差异表达。为了确定这些基因在红品种果实成熟后期是否遵循相似的表达模式，选择了9个基因进行RT-PCR分析，赤霞珠生长在两种不同温度条件下影响果实早熟。赤霞珠中的7个基因的表达谱及其与成熟的关系得到了证实，它们分别编码类胡萝卜素裂解双加氧酶、半乳糖醇合成酶、胚胎发育后期丰富的蛋白、类似生物的蛋白、组氨酸激酶受体、戊烯合酶和可能的S-腺苷-L-蛋氨酸：水杨酸羧甲基转移酶。这组上调和下调的基因描述了两个被研究品种的浆果成熟后期的特征，并间接与葡萄酒质量有关。它们可以直接或间接地用于设计免疫学、生化或分子工具，以确定这些品种的最佳成熟度。
The composition of grapevine berry at harvest is a major determinant of wine quality. Optimal oenological maturity of berries is characterized by a high sugar/acidity ratio, high anthocyanin content in the skin, and low astringency. However, harvest time is still mostly determined empirically, based on crude biochemical composition and berry tasting. In this context, it is interesting to identify genes that are expressed/repressed specifically at the late stages of ripening and which may be used as indicators of maturity. Whole bunches and berries sorted by density were collected in vineyard on Chardonnay (white cultivar) grapevines for two consecutive years at three stages of ripening (7-days before harvest (TH-7), harvest (TH), and 10-days after harvest (TH+10)). Microvinification and sensory analysis indicate that the quality of the wines made from the whole bunches collected at TH-7, TH and TH+10 differed, TH providing the highest quality wines. In parallel, gene expression was studied with Qiagen/Operon microarrays using two types of samples, i.e. whole bunches and berries sorted by density. Only 12 genes were consistently up- or down-regulated in whole bunches and density sorted berries for the two years studied in Chardonnay. 52 genes were differentially expressed between the TH-7 and TH samples. In order to determine whether these genes followed a similar pattern of expression during the late stages of berry ripening in a red cultivar, nine genes were selected for RT-PCR analysis with Cabernet Sauvignon grown under two different temperature regimes affecting the precocity of ripening. The expression profiles and their relationship to ripening were confirmed in Cabernet Sauvignon for seven genes, encoding a carotenoid cleavage dioxygenase, a galactinol synthase, a late embryogenesis abundant protein, a dirigent-like protein, a histidine kinase receptor, a valencene synthase and a putative S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase. This set of up- and down-regulated genes characterize the late stages of berry ripening in the two cultivars studied, and are indirectly linked to wine quality. They might be used directly or indirectly to design immunological, biochemical or molecular tools aimed at the determination of optimal ripening in these cultivars.
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影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
6.4
作者：
Chourasia, Amita;Sane, Vidhu A.;Nath, Pravendra
通讯作者：
Nath, Pravendra
影响因子：
7.4
作者：
Davies, C;Boss, PK;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
7.4
作者：
Davies, C;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
6.2
作者：
Baumes, R;Wirth, J;Razungles, A
通讯作者：
Razungles, A