Construction of chromosome segment substitution lines enables QTL mapping for flowering and morphological traits in Brassica rapa.
Construction of chromosome segment substitution lines enables QTL mapping for flowering and morphological traits in Brassica rapa.
复制标题
染色体片段替换系的构建可实现白菜开花和形态性状的 QTL 定位
DOI：
10.3389/fpls.2015.00432
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Li X;Wang W;Wang Z;Li K;Lim YP;Piao Z
染色体片段替代系（CSSLS）是精确检测植物复杂农艺性状的数量性状基因座（QTL）的有力方法。在这项研究中，我们使用了一个标记辅助回交策略，以开发一个由63个CSSLs组成的群体，来自两个芜菁亚种“Chiifu”（ssp. pekinensis）、用作供体的芸苔属“A”基因组代表系和“49菜心”（ssp. parachinensis），一种用作受体的不结球品种。63个CSSL覆盖了B的87.95%。芜菁基因组其中，39个品系携带供体亲本染色体的一个片段，15个品系携带供体亲本染色体的两个片段，9个品系携带供体亲本染色体的三个或更多片段。为了验证这些CSSL系的潜在优势，我们利用它们定位了6个形态相关性状的QTL。在8条染色体上共定位到58个QTL，其中开花时间17个，抽薹时间和株高各14个，株径6个，叶宽2个，花茎粗5个。共定位的QTL主要分布在A01、A02、A05、A06、A08、A09和A10的8个基因组区域上，存在于相应的CSSLs中。此外，新的染色体片段，窝藏QTL被确定使用以前的研究结果。本研究构建的CSSL群体为B中与晚抽薹、开花和植株构型相关性状的候选基因的精细定位和克隆奠定了基础。拉帕。此外，它具有很大的潜力，未来的标记辅助基因/QTL的其他感兴趣的性状在B。芜菁育种
Chromosome segment substitution lines (CSSLs) represent a powerful method for precise quantitative trait loci (QTL) detection of complex agronomical traits in plants. In this study, we used a marker-assisted backcrossing strategy to develop a population consisting of 63 CSSLs, derived from backcrossing of the F1 generated from a cross between two Brassica rapa subspecies: “Chiifu” (ssp. pekinensis), the Brassica “A” genome-represented line used as the donor, and “49caixin” (ssp. parachinensis), a non-heading cultivar used as the recipient. The 63 CSSLs covered 87.95% of the B. rapa genome. Among them, 39 lines carried a single segment; 15 lines, two segments; and nine lines, three or more segments of the donor parent chromosomes. To verify the potential advantage of these CSSL lines, we used them to locate QTL for six morphology-related traits. A total of 58 QTL were located on eight chromosomes for all six traits: 17 for flowering time, 14 each for bolting time and plant height, six for plant diameter, two for leaf width, and five for flowering stalk diameter. Co-localized QTL were mainly distributed on eight genomic regions in A01, A02, A05, A06, A08, A09, and A10, present in the corresponding CSSLs. Moreover, new chromosomal fragments that harbored QTL were identified using the findings of previous studies. The CSSL population constructed in our study paves the way for fine mapping and cloning of candidate genes involved in late bolting, flowering, and plant architecture-related traits in B. rapa. Furthermore, it has great potential for future marker-aided gene/QTL pyramiding of other interesting traits in B. rapa breeding.
登录
查看更多内容
影响因子：
3.7
作者：
Fonceka D;Tossim HA;Rivallan R;Vignes H;Lacut E;de Bellis F;Faye I;Ndoye O;Leal-Bertioli SC;Valls JF;Bertioli DJ;Glaszmann JC;Courtois B;Rami JF
通讯作者：
Rami JF
影响因子：
1.9
作者：
Miura, H;Wickramasinghe, MHA;Komae, K
通讯作者：
Komae, K
影响因子：
3.1
作者：
Li, Xiaonan;Ramchiary, Nirala;Lim, Yong Pyo
通讯作者：
Lim, Yong Pyo
影响因子：
2.4
作者：
Ge, Yu;Ramchiary, Nirala;Piao, Zhong Yun
通讯作者：
Piao, Zhong Yun
影响因子：
5.4
作者：
Muangprom, A;Osborn, TC
通讯作者：
Osborn, TC