Characterization of the reproductive tract bacterial microbiota of virgin, mated, and blood-fed Aedes aegypti and Aedes albopictus females.
Characterization of the reproductive tract bacterial microbiota of virgin, mated, and blood-fed Aedes aegypti and Aedes albopictus females.
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DOI：
10.1186/s13071-021-05093-7
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发表时间：
2021-12-01
影响因子：
3.2
通讯作者：
Avila FW
中科院分区：
文献类型：
--
作者：
Díaz S;Camargo C;Avila FW
埃及伊蚊Aedes aegypti和Ae.白纹伊蚊是许多虫媒病毒的载体，对人类健康有不利影响。在疾病的蚊子媒介中，细菌微生物群影响几个生理过程，包括生育力和媒介能力，使得操纵细菌群落成为控制蚊子媒介的有希望的方法。在这项研究中，我们描述了生殖道组织微生物群的实验室饲养的处女Ae。埃及伊蚊和埃及伊蚊。白纹伊蚊雄性和每个物种的未交配、交配和交配+吸血雌性，将在那里发现的细菌组成与充分描述的肠道微生物群进行比较。我们对从每个物种的肠道、上生殖道（URT;睾丸或卵巢）和下生殖道（LRT;雄性：精囊和附属腺;雌性：输卵管、受精囊和囊）分离的16 S rRNA进行了元条形码编码，并评估了宿主物种、组织、营养状态和生殖状态对微生物群组成的影响。最后，基于所评估的组织的鉴定的分类学概况，预测细菌代谢途径丰度。与肠道相比，生殖道的群落结构是独特的。Asaia是两个Ae的LRT中最普遍的OTU。埃及伊蚊和埃及伊蚊。白纹伊蚊在URT中，我们观察到物种之间的差异，Wolbachia OTUs在Ae中占主导地位。白纹伊蚊URT占优势，而肠杆菌属和沙雷氏菌属在Ae.埃及URT.与生殖状态相比，宿主物种和组织是群落组成的最佳预测因子（即，处女或交配）和营养状况（即，糖或血液喂养的）。预测的功能概况显示了与交配和吸血相关的特定微生物途径丰度的变化，如交配组织中的能量产生和吸血雌性组织中的铁载体合成。埃及伊蚊Aedes aegypti和Ae.白纹伊蚊在生殖道中发现的微生物群的组成方面存在明显差异。细菌分类群的分布表明，一些细菌对生殖道组织具有组织特异性嗜性，如Asaia和Wolbachia。未观察到处女，交配，交配+血液喂养的女性之间的生殖道的分类组成的显着差异，但在特定的代谢途径的丰度的变化被发现在交配和血液喂养的女性预测的微生物功能配置文件。在线版本包含补充材料，可通过10.1186/s13071-021-05093-7获得。
Aedes aegypti and Ae. albopictus are vectors of numerous arboviruses that adversely affect human health. In mosquito vectors of disease, the bacterial microbiota influence several physiological processes, including fertility and vector competence, making manipulation of the bacterial community a promising method to control mosquito vectors. In this study, we describe the reproductive tract tissue microbiota of lab-reared virgin Ae. aegypti and Ae. albopictus males, and virgin, mated, and mated + blood-fed females of each species, comparing the bacterial composition found there to the well-described gut microbiota. We performed metabarcoding of the 16S rRNA isolated from the gut, upper reproductive tract (URT; testes or ovaries), and lower reproductive tract (LRT; males: seminal vesicles and accessory glands; females: oviduct, spermathecae, and bursa) for each species, and evaluated the influence of host species, tissue, nutritional status, and reproductive status on microbiota composition. Finally, based on the identified taxonomic profiles of the tissues assessed, bacterial metabolic pathway abundance was predicted. The community structure of the reproductive tract is unique compared to the gut. Asaia is the most prevalent OTU in the LRTs of both Ae. aegypti and Ae. albopictus. In the URT, we observed differences between species, with Wolbachia OTUs being dominant in the Ae. albopictus URT, while Enterobacter and Serratia were dominant in Ae. aegypti URT. Host species and tissue were the best predictors of the community composition compared to reproductive status (i.e., virgin or mated) and nutritional status (i.e., sugar or blood-fed). The predicted functional profile shows changes in the abundance of specific microbial pathways that are associated with mating and blood-feeding, like energy production in mated tissues and siderophore synthesis in blood-fed female tissues. Aedes aegypti and Ae. albopictus have distinct differences in the composition of microbiota found in the reproductive tract. The distribution of the bacterial taxonomic groups indicates that some bacteria have tissue-specific tropism for reproductive tract tissue, such as Asaia and Wolbachia. No significant differences in the taxonomic composition were observed in the reproductive tract between virgin, mated, and mated + blood-fed females, but changes in the abundance of specific metabolic pathways were found in the predicted microbial functional profiles in mated and blood-fed females. The online version contains supplementary material available at 10.1186/s13071-021-05093-7.
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影响因子：
4.2
作者：
Chouaia B;Rossi P;Epis S;Mosca M;Ricci I;Damiani C;Ulissi U;Crotti E;Daffonchio D;Bandi C;Favia G
通讯作者：
Favia G
影响因子：
4.8
作者：
Díaz S;Escobar JS;Avila FW
通讯作者：
Avila FW
影响因子：
3.2
作者：
Dickson, Laura B.;Ghozlane, Amine;Lambrechts, Louis
通讯作者：
Lambrechts, Louis
影响因子：
4.6
作者：
Bassene, Hubert;Niang, El Hadji Amadou;Mediannikov, Oleg
通讯作者：
Mediannikov, Oleg
影响因子：
4.6
作者：
Camargo, Carolina;Ahmed-Braimah, Yasir H.;Avila, Frank W.
通讯作者：
Avila, Frank W.