The integrative taxonomic approach reveals host specific species in an encyrtid parasitoid species complex.
The integrative taxonomic approach reveals host specific species in an encyrtid parasitoid species complex.
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综合分类方法揭示了寄生蜂物种复合体中的寄主特定物种
DOI：
10.1371/journal.pone.0037655
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang YZ
中科院分区：
文献类型：
--
作者：
Chesters D;Wang Y;Yu F;Bai M;Zhang TX;Hu HY;Zhu CD;Li CD;Zhang YZ
综合分类学使用来自许多不同特征类型的证据来划分物种和其他自然群体。虽然这种方法最近一直提倡，并应在小型昆虫寄生蜂的情况下，特别实用，有相对较少的例子，它的应用在这些类群。在这里，我们使用一个综合的框架来划定独立的谱系在Enachtus sasakii（Hyplutera：Chalcidoidea：Enachtididae），寄生形态种以前被认为是一个主机通才。对分布于中国北方和华中地区的4种蚧虫进行了形态学和交配亲和性测定，并对DNA条形码（细胞色素c氧化酶I，COI）和核28SrDNA位点的序列变异进行了比较。我们的研究结果表明，Enachtus sasakii包括三个谱系，虽然共享一个相似的形态，是高度分歧的分子水平。在条形码基因座，从三个主要人群的个体之间的中位数K2P分子距离被认为是11.3%，远超出通常观察到的分歧小蜂总科同种（0.5%）。确凿的证据表明，遗传谱系代表独立的物种被发现从交配测试，其中只观察到种群内的兼容性，和形态测量分析，发现，尽管明显的形态同质性，种群集群根据前翅形状。通过综合分析确定的独立谱系对应于三种介壳虫宿主，表明存在宿主特异性隐种。在这个物种复合体中发现隐藏的宿主特异性表明，DNA条形码在揭示传统分类方法难以实现的分类群中隐藏的生物多样性方面将发挥越来越重要的作用。
Integrated taxonomy uses evidence from a number of different character types to delimit species and other natural groupings. While this approach has been advocated recently, and should be of particular utility in the case of diminutive insect parasitoids, there are relatively few examples of its application in these taxa. Here, we use an integrated framework to delimit independent lineages in Encyrtus sasakii (Hymenoptera: Chalcidoidea: Encyrtidae), a parasitoid morphospecies previously considered a host generalist. Sequence variation at the DNA barcode (cytochrome c oxidase I, COI) and nuclear 28S rDNA loci were compared to morphometric recordings and mating compatibility tests, among samples of this species complex collected from its four scale insect hosts, covering a broad geographic range of northern and central China. Our results reveal that Encyrtus sasakii comprises three lineages that, while sharing a similar morphology, are highly divergent at the molecular level. At the barcode locus, the median K2P molecular distance between individuals from three primary populations was found to be 11.3%, well outside the divergence usually observed between Chalcidoidea conspecifics (0.5%). Corroborative evidence that the genetic lineages represent independent species was found from mating tests, where compatibility was observed only within populations, and morphometric analysis, which found that despite apparent morphological homogeneity, populations clustered according to forewing shape. The independent lineages defined by the integrated analysis correspond to the three scale insect hosts, suggesting the presence of host specific cryptic species. The finding of hidden host specificity in this species complex demonstrates the critical role that DNA barcoding will increasingly play in revealing hidden biodiversity in taxa that present difficulties for traditional taxonomic approaches.
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影响因子：
6.5
作者：
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通讯作者：
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