Gut microbiome in adolescent depression.
Gut microbiome in adolescent depression.
复制标题
DOI：
10.1016/j.jad.2021.05.107
复制
发表时间：
2021-09-01
影响因子：
6.6
通讯作者：
Calarge CA
中科院分区：
文献类型：
--
作者：
Thapa S;Sheu JC;Venkatachalam A;Runge JK;Luna RA;Calarge CA
研究重度抑郁症 (MDD) 和选择性血清素再摄取抑制剂 (SSRI) 的使用与老年青少年和年轻人肠道微生物组的关系。开始服用 SSRI 一个月内的 15 至 20 岁参与者和未服用药物的对照组参加了一项纵向研究。他们接受了诊断评估，包括自行填写和评估者管理的问卷以及临床访谈。他们还提供了粪便样本，在-80°C 下保存直至提取 DNA。使用MoBio PowerSoil试剂盒提取微生物DNA，并对16S rRNA的V4区进行扩增和测序。原始序列数据使用 LotuS 管道进行处理。分析中仅包含过去 6 个月内未接触过抗生素且具有 >1000 个质量过滤读数的样本。 160 名参与者（57.5% 为女性，平均年龄 20.0±1.9 岁，29% 服用 SSRIs）参与研究，其中包括 110 名 MDD 患者（60% 为急性发作）、27 名健康对照者和 23 名精神病对照者。在细菌丰富度或α和β多样性方面没有观察到显着的组间差异。细菌分类群的差异丰度分析发现，门和属水平上没有显着的群体差异。无论是处于重度抑郁发作还是缓解状态，还是使用 SSRIs，都与细菌组成的差异无关。在这一大量老年青少年样本中，MDD 和 SSRI 的使用均与肠道细菌微生物组的差异无关。在这个年龄段，肠道细菌和大脑之间的双向相互作用可能比成年人更加微妙，需要进一步研究。
To examine the association of major depressive disorder (MDD) and selective serotonin reuptake inhibitor (SSRI) use with gut microbiome in older adolescents and younger adults. Fifteen to 20-year-old participants within a month of starting an SSRI and unmedicated controls were enrolled in a longitudinal study. They underwent a diagnostic evaluation comprising self-completed and rater-administered questionnaires and clinical interview. They also provided a stool sample, which was stored at −80°C until DNA extraction. Microbial DNA was extracted with the MoBio PowerSoil kit, and the V4 region of the 16S rRNA was amplified and sequenced. Raw sequence data was processed with the LotuS pipeline. Only samples with no antibiotic exposure in the last 6 months and with >1000 quality filtered reads were included in the analysis. 160 participants (57.5% female, mean age 20.0±1.9 years, 29% taking SSRIs) were enrolled, comprising 110 MDD patients (60% in acute episode), 27 healthy controls, and 23 psychiatric controls. No significant group differences were observed in bacterial richness or alpha and beta diversity. Differential abundance analysis of bacterial taxa found no significant group differences at the phylum and genus levels. Neither being in a major depressive episode vs. remission nor using SSRIs was associated with differential bacterial composition. In this sizeable sample of older adolescents, neither MDD nor SSRI use was associated with differences in gut bacterial microbiome. In this age group, the bi-directional interaction between the gut bacteria and brain may be more nuanced than in adults, requiring further investigation.
登录
查看更多内容
影响因子：
15.5
作者：
Hildebrand F;Tadeo R;Voigt AY;Bork P;Raes J
通讯作者：
Raes J
益生菌对抑郁症的影响：随机对照试验的系统回顾和荟萃分析
影响因子：
5.9
作者：
Huang R;Wang K;Hu J
通讯作者：
Hu J
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1016/j.pnpbp.2015.06.013
发表时间：
2016-02-04
影响因子：
5.6
作者：
Gorky J;Schwaber J
通讯作者：
Schwaber J
影响因子：
15.1
作者：
Jiang, Haiyin;Ling, Zongxin;Ruan, Bing
通讯作者：
Ruan, Bing