Development of a novel fluorophore for real-time biomonitoring system.
Development of a novel fluorophore for real-time biomonitoring system.
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DOI：
10.1371/journal.pone.0048459
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Park H
中科院分区：
文献类型：
--
作者：
Song HO;Lee B;Bhusal RP;Park B;Yu K;Chong CK;Cho P;Kim SY;Kim HS;Park H
现场快速诊断对于预防各种传染病和接触性疾病的爆发非常重要。使用具有特异性抗原-抗体结合和高质量荧光团的荧光免疫化学测定可以进行疾病的高灵敏度和定量检测。这可能导致开发小型、便携式、定量生物传感器，将诊断结果传输到控制中心，以系统地预防疾病爆发。在这项研究中，我们开发了一种新的荧光团，香豆素衍生的树枝状大分子，具有高发射强度，强信号亮度，和高的光稳定性。它很容易与生物分子偶联，并在455 nm激发下在590 nm处发出强烈而稳定的荧光。应用荧光免疫层析试验（FICT）表明，新的香豆素衍生的树枝状大分子生物共轭物可以检测抗原的量低至0.1 ng。新型香豆素衍生的树枝状聚合物的临床结果和光谱特性首次开启了开发用于护理点（POC）疾病诊断的基于LED的成本/能量有效的便携式定量生物传感器的可能性，其可以允许疾病控制中心进行真实的时间监测（U-医疗保健系统）。
Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic results to a control center in order to systematically prevent disease outbreaks. In this study, we developed a novel fluorophore, coumarin-derived dendrimer, with high emission intensity, strong signal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590 nm with excitation at 455 nm. Application to fluorescent immunochromatographic test (FICT) showed that the novel coumarin-derived dendrimer bioconjugate could detect antigens at amount as low as 0.1 ng. The clinical results and the spectral characteristics of the novel coumarin-derived dendrimer open, for the first time, the possibility of developing a cost/energy efficient LED-based portable quantitative biosensor for point-of-care (POC) disease diagnosis, which can permit real time monitoring (U-healthcare system) by a disease control center.
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