Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities.
Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities.
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不同强度静磁场对MC3T3-E1细胞成骨细胞分化和铁含量的调控
DOI：
10.1007/s12011-017-1161-5
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发表时间：
2018-07
影响因子：
3.9
通讯作者：
Shang P
中科院分区：
文献类型：
--
作者：
Yang J;Zhang J;Ding C;Dong D;Shang P
许多研究表明，静磁场（SMFs）对骨组织有积极的影响，包括骨形成和骨愈合过程。评估SMFs对骨细胞（尤其是成骨细胞）功能的影响并探索其机制，对于了解SMFs对骨重塑平衡的可能风险或益处至关重要。铁与磁场有着天然的关系，铁是正常骨代谢所必需的元素。铁超载或缺铁会导致严重的骨骼疾病，包括骨质疏松症。然而，关于铁在smf下调节骨形成中的作用的报道很少。本研究采用500 nT的低磁场（HyMF）、0.2 T的中等强磁场（MMF）和16 T的高强磁场（HiMF）来研究成骨细胞MC3T3-E1对强磁场的反应以及在强磁场作用下成骨细胞铁代谢的变化。结果表明，SMFs对成骨细胞生长没有严重的毒性作用。在细胞增殖过程中，HyMF降低了成骨细胞MC3T3-E1细胞的铁含量，但暴露48 h后MMF和MMF的铁含量升高。与未处理对照（即地磁场，GMF）相比，HyMF和MMF通过同时延缓碱性磷酸酶（ALP）活性、矿化和钙沉积，对成骨细胞分化产生有害影响。然而，当暴露于16 T的HiMF时，分化电位呈现相反的趋势，矿化增强。在细胞分化过程中，铁水平在HyMF中升高，在MMF中保持不变，在HiMF中降低。此外，HyMF可促进转铁蛋白受体1 (TFR1) mRNA的表达，而HiMF可抑制其表达。同时，16 T的HiMF和0.2 T的MMF增加了铁转运蛋白1 （FPN1）的表达。综上所述，这些结果表明成骨细胞的分化可以通过改变SMF的强度来调节，而铁可能参与了这一过程。
Many studies have indicated that static magnetic fields (SMFs) have positive effects on bone tissue, including bone formation and bone healing process. Evaluating the effects of SMFs on bone cell (especially osteoblast) function and exploring the mechanism, which is critical for understanding the possible risks or benefits from SMFs to the balance of bone remodeling. Iron and magnetic fields have the natural relationship, and iron is an essential element for normal bone metabolism. Iron overload or deficiency can cause severe bone disorders including osteoporosis. However, there are few reports regarding the role of iron in the regulation of bone formation under SMFs. In this study, hypomagnetic field (HyMF) of 500 nT, moderate SMF (MMF) of 0.2 T, and high SMF (HiMF) of 16 T were used to investigate how osteoblast (MC3T3-E1) responses to SMFs and iron metabolism of osteoblast under SMFs. The results showed that SMFs did not pose severe toxic effects on osteoblast growth. During cell proliferation, iron content of osteoblast MC3T3-E1 cells was decreased in HyMF, but was increased in MMF and HiMF after exposure for 48 h. Compared to untreated control (i.e., geomagnetic field, GMF), HyMF and MMF exerted deleterious effects on osteoblast differentiation by simultaneously retarding alkaline phosphatase (ALP) activity, mineralization and calcium deposition. However, when exposed to HiMF of 16 T, the differentiation potential showed the opposite tendency with enhanced mineralization. Iron level was increased in HyMF, constant in MMF and decreased in HiMF during cell differentiation. In addition, the mRNA expression of transferrin receptor 1 (TFR1) was promoted by HyMF but was inhibited by HiMF. At the same time, HiMF of 16 T and MMF of 0.2 T increased the expression of ferroportin 1 (FPN1). In conclusion, these results indicated that osteoblast differentiation can be regulated by altering the strength of the SMF, and iron is possibly involved in this process.
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影响因子：
14
作者：
Ba, Xiaolan;Hadjiargyrou, Michael;Rafailovich, Miriam H.
通讯作者：
Rafailovich, Miriam H.
影响因子：
10.1
作者：
Baschant, Ulrike;Rauner, Mar Tina;Hofbauer, Lorenz C.
通讯作者：
Hofbauer, Lorenz C.
影响因子：
3.8
作者：
Chiu, Kang-Hsuan;Ou, Keng-Liang;Huang, Haw-Ming
通讯作者：
Huang, Haw-Ming
影响因子：
3.7
作者：
Jia B;Xie L;Zheng Q;Yang PF;Zhang WJ;Ding C;Qian AR;Shang P
通讯作者：
Shang P
DOI：
10.1097/01.blo.0000203464.35561.be
发表时间：
2006-06-01
影响因子：
4.2
作者：
Huang, Haw-Ming;Lee, Sheng-Yang;Yeh, Ching-Ying
通讯作者：
Yeh, Ching-Ying