Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer.
Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer.
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木防己汤及其活性成分广藿香醇通过调节Akt/mTOR介导的自噬抑制非小细胞肺癌肿瘤生长
DOI：
10.1155/2021/2373865
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Yang L;Chen H;Li R;Li H;Rui X;Zhou L;Liu N;Ji Q;Li Q
非小细胞肺癌（non-small cell lung cancer，NSCLC）是肺癌的主要类型，其发病率和死亡率均居恶性肿瘤之首。70%以上的NSCLC患者确诊时已是局部晚期或中晚期，错过了最佳手术期。化疗和靶向治疗是治疗晚期非小细胞肺癌的重要手段，但各种毒副作用严重影响了治疗效果和患者的生活质量。我们前期的临床实践表明，经典中药木防己汤治疗非小细胞肺癌疗效显著，但具体作用机制尚不清楚。本研究旨在探讨木防己汤及其有效成分广藿香醇抗NSCLC的潜在机制，为中医药防治NSCLC提供科学依据。 通过体内外实验，评价木防己汤及其有效成分广藿香醇的抗肿瘤作用，并探讨其作用机制。运用网络药理学方法分析木防己汤的有效成分和潜在作用靶点或信号通路。 本研究表明木防己汤能有效抑制皮下移植的非小细胞肺癌的生长。以下网络药理分析和体内实验提示广藿香醇是木防己汤的主要活性成分之一，具有抗肿瘤作用。进一步的机制研究表明，广藿香醇的抗肿瘤作用与诱导Akt/mTOR信号通路介导的NSCLC细胞自噬有关。 木防己汤及其有效成分广藿香醇可能部分通过调节Akt/mTOR介导的自噬而发挥抗NSCLC作用，为中医药通过靶向Akt/mTOR信号轴治疗NSCLC提供了证据。
Nonsmall-cell lung cancer (NSCLC) is the main type of lung cancer, whose morbidity and mortality rank first among malignant tumors. More than 70% of NSCLC patients are diagnosed at locally advanced or advanced stage, missing the best operation period. Chemotherapy and targeted therapy are important means for the treatment of advanced NSCLC, but various side effects seriously affect the curative effect and the life quality of NSCLC patients. Our previous clinical practice has shown that Mufangji Decoction, a classic traditional Chinese medicine, has a significant curative effect in the treatment of NSCLC, but the specific mechanism is not clear. This study intends to explore the potential mechanism of Mufangji Decoction and its active ingredient patchouli alcohol against NSCLC and to provide a scientific basis for the prevention and treatment of NSCLC by traditional Chinese medicine. The in vivo and in vitro experiments were performed to evaluate the antitumor effects and investigate the underlying mechanism of Mufangji Decoction and its active ingredient patchouli alcohol. Network pharmacology was applied to analyze the effective ingredients and potential targets or signaling pathways of Mufangji Decoction. Our current study shows that Mufangji Decoction can effectively inhibit the growth of subcutaneous transplantation of NSCLC. The following network pharmacological analysis and in vivo experiment suggest that patchouli alcohol is one of the main active ingredients of Mufangji Decoction and exerts antitumor effects. Further mechanism investigation reveals that the antitumor effect of patchouli alcohol is related to the induction of Akt/mTOR signaling pathway-mediated autophagy in NSCLC cells. Mufangji Decoction and its active ingredient patchouli alcohol might exert their antitumor effects in NSCLC partly through regulating Akt/mTOR-mediated autophagy, providing the evidence that traditional Chinese medicine might be a key approach for NSCLC treatment via targeting the Akt/mTOR signal axis.
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