Pleiotropic Effects of Rice Florigen Gene RFT1 on the Amino Acid Content of Unmilled Rice
Pleiotropic Effects of Rice Florigen Gene RFT1 on the Amino Acid Content of Unmilled Rice
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水稻成花基因RFT1对糙米氨基酸含量的多效性影响
DOI：
10.3389/fgene.2020.00013
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发表时间：
2020-01
影响因子：
3.7
通讯作者：
Jie-Yun Zhuang
中科院分区：
文献类型：
--
作者：
Li-Hong Xie;Yu-Jun Zhun;Shao-Qing Tang;Xiang-Jin Wei;Zhong-Hua Sheng;Gui-Ai Jiao;Pei-Song Hu;Jie-Yun Zhuang
在大米中，蛋白质和氨基酸的含量是衡量大米营养品质的主要参数。抽穗期和蛋白质含量的数量性状基因(QTL)共定位已有报道，但抽穗期基因对蛋白质含量的多效性尚未见报道。本文报道了水稻成花素基因RFT1在控制水稻籽粒氨基酸含量中的重要作用。首先，利用水稻重组自交系珍山97(ZS97)/密阳46(My46)检测到73个控制精米中17种氨基酸含量的QTL。然后，利用只分离了一个覆盖目标QTL的区域的近等基因系(NILs)组成的三个群体，验证了位于水稻成花基因RFT1和Hd3a附近的由14个QTL组成的最大簇的效果。第一个和第二个近等基因系是从ZS97/My46 RIL群体中鉴定的一个残留杂合子衍生而来的，这些纯合子系只分离在覆盖两个成花基因的29.9kb区域和RFT1的1.7kb区域。以中花11号为背景，分离了RFT1 ZS97转基因的第3个近等位群体。在所有3个近等位群体中，RFT1对大多数氨基酸的含量都有很强的影响，但ZS97等位基因总是起到降低作用。通过比较在ZS97/My46 RIL群体中检测到的氨基酸含量的QTL和ZS97与My46抽穗期差异的基因/QTL，还发现其他关键抽穗期基因hd1、Ghd7和OSGi也可能对氨基酸含量产生多效性。
In rice, the contents of protein and amino acids are the major parameters of nutritional quality. Co-localization of quantitative trait loci (QTLs) for heading date and protein content were reported, but pleiotropism of heading-date genes on protein contents has not been investigated. Here, we reported that rice florigen gene RFT1 plays an important role in controlling amino acid contents of rice grain. Firstly, 73 QTLs for the contents of 17 amino acids in unmilled rice were detected using recombinant inbred lines (RILs) of the indica rice cross Zhenshan 97 (ZS97)/Milyang 46 (MY46). Then, the effect of the largest cluster consisting of 14 QTLs, located in proximity to the rice florigen genes RFT1 and Hd3a, was validated using three populations consisting of near isogenic lines (NILs) that only segregated a region covering the target QTL. The first and second NIL populations were derived from a residual heterozygote identified from the ZS97/MY46 RIL population, consisting of homozygous lines that were only segregated in a 29.9-kb region covering the two florigen genes and a 1.7-kb region for RFT1, respectively. The third NIL population was segregated for the RFT1 ZS97 transgene in the background of japonica rice cultivar Zhonghua 11. In all the three NIL populations, RFT1 was shown to have a strong effect on the contents of most amino acids, with the ZS97 allele always having the reducing effects. By comparing QTLs for amino acid contents detected in the ZS97/MY46 RIL population and genes/QTLs previously identified for heading date difference between ZS97 and MY46, possible pleiotropism on amino acid contents was also shown for other key heading-date genes including Hd1, Ghd7, and OsGI.
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DOI：
10.1186/s12284-016-0135-0
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Yun YT;Chung CT;Lee YJ;Na HJ;Lee JC;Lee SG;Lee KW;Yoon YH;Kang JW;Lee HS;Lee JY;Ahn SN
通讯作者：
Ahn SN
影响因子：
4.6
作者：
Zhu YJ;Fan YY;Wang K;Huang DR;Liu WZ;Ying JZ;Zhuang JY
通讯作者：
Zhuang JY
影响因子：
2.4
作者：
Wada, Takuya;Uchimura, Yosuke;Matsue, Yuji
通讯作者：
Matsue, Yuji
影响因子：
4.1
作者：
Lu, Kaiyang;Li, Lanzhi;Hu, Zhongli
通讯作者：
Hu, Zhongli
影响因子：
2.4
作者：
Yoshida, S;Ikegami, M;Kamijima, O
通讯作者：
Kamijima, O