Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica).
Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica).
复制标题
杂交水稻（Oryza sativa L. ssp. indica）叶色基因 Ygl7 的定位克隆及功能分析。
DOI：
10.1371/journal.pone.0099564
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang GL
中科院分区：
文献类型：
--
作者：
Deng XJ;Zhang HQ;Wang Y;He F;Liu JL;Xiao X;Shu ZF;Li W;Wang GH;Wang GL
叶片颜色是鉴定水稻杂交品种的有效标记。叶片颜色相关基因在高等植物叶绿体发育和光合色素生物合成中起着重要作用。ygl7 （yellow-green leaf 7）是一种全寿命自发黄绿叶表型突变体，其产量性状没有变化。我们克隆了编码镁螯合酶ChlD蛋白的基因Ygl7 （Os03g59640）。ygl7的表达使绿叶变成黄色，而rnai介导的ygl7沉默导致转基因植株的致死表型。这说明了该基因对水稻植株的重要性。另一方面，它证实了ygl7是一个非零突变体。Ygl7的光合色素含量低于野生型，但光效较高。这些结果表明，突变的YGL7蛋白不会导致原有功能的完全丧失，而是作为一个新的蛋白执行新的功能。这种新功能部分包含了其先前的功能，并具有促进光合作用的附加功能。ch1， Ygl98和Ygl3是先前记录的OsChlD基因的三个等位基因。然而，三种突变体中OsChlD突变基因的突变位点及其编码蛋白产物不同。这三种突变体抑制了粮食产量。在我们的实验中，三个突变体（ygl7、chl1和ygl98）的植株材料在整个生长时期都表现出突变的叶色。这一结果与以往的研究有所不同。我们将ygl7分别与chl1和ygl98杂交。F1和F2代均表现为黄绿叶表型，叶绿素和类胡萝卜素含量在亲本之间。此外，我们注意到一个重要的现象：ygl7- nil的叶片颜色是黄色的，而不是黄绿色的，所有与ygl7回交的后代也是如此。
Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F1 and F2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.
登录
查看更多内容
影响因子：
7.4
作者：
Guo, RB;Luo, MZ;Weinstein, JD
通讯作者：
Weinstein, JD
影响因子：
4.8
作者：
Huang, Xiao-qun;Wang, Ping-rong;Deng, Xiao-jian
通讯作者：
Deng, Xiao-jian
影响因子：
5.1
作者：
Gothandam, KM;Kim, ES;Chung, YY
通讯作者：
Chung, YY
影响因子：
2.7
作者：
Hibara, Ken-ichiro;Obara, Mari;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
影响因子：
3.1
作者：
Masuda, Tatsuru;Fujita, Yuichi
通讯作者：
Fujita, Yuichi