A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
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DOI：
10.1371/journal.pone.0029317
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
van der Hoorn RA
中科院分区：
文献类型：
--
作者：
Shindo T;Misas-Villamil JC;Hörger AC;Song J;van der Hoorn RA
分泌型木瓜蛋白酶样半胱氨酸蛋白酶在植物免疫中起重要作用。我们曾报道番茄的C14蛋白是由卵菌致病疫霉(Phytophthora infestans，Pinf)在侵染过程中分泌的类胱抑素EPIC蛋白的靶标。在与Pinf共同进化的野生马铃薯物种中，C14一直处于多样化的选择之下，C14水平的降低导致对Pinf的敏感性增加。在这里，我们研究了C14-EPIC类相互作用在拟南芥与卵菌病原菌阿拉伯透明盘孢菌(HPA)自然病理系统中的作用。与Pinf-Solanaceae的病理系统相反，拟南芥的C14同源蛋白酶RD21在拟南芥中不会在多样化选择下进化，并且rd21缺失突变体在亲和和不亲和的HPA相互作用中不表现出表型，尽管明显缺乏主要的叶片蛋白酶。HPA分离株在感染过程中表达高度保守的EPIC样蛋白，但尚不清楚这些Hpa EPIC是否能抑制RD21，其中一个Hpa EPIC甚至缺乏典型的半胱氨酸氨基转移酶(Cystatin)基序。Rd21突变体在与丁香假单胞菌的亲和和不亲和相互作用中不受影响。但对坏死性真菌病原菌灰霉病菌更敏感，这表明RD21对坏死性病原菌具有免疫力。
Secreted papain-like Cys proteases are important players in plant immunity. We previously reported that the C14 protease of tomato is targeted by cystatin-like EPIC proteins that are secreted by the oomycete pathogen Phytophthora infestans (Pinf) during infection. C14 has been under diversifying selection in wild potato species coevolving with Pinf and reduced C14 levels result in enhanced susceptibility for Pinf. Here, we investigated the role C14-EPIC-like interactions in the natural pathosystem of Arabidopsis with the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa). In contrast to the Pinf-solanaceae pathosystem, the C14 orthologous protease of Arabidopsis, RD21, does not evolve under diversifying selection in Arabidopsis, and rd21 null mutants do not show phenotypes upon compatible and incompatible Hpa interactions, despite the evident lack of a major leaf protease. Hpa isolates express highly conserved EPIC-like proteins during infections, but it is unknown if these HpaEPICs can inhibit RD21 and one of these HpaEPICs even lacks the canonical cystatin motifs. The rd21 mutants are unaffected in compatible and incompatible interactions with Pseudomonas syringae pv. tomato, but are significantly more susceptible for the necrotrophic fungal pathogen Botrytis cinerea, demonstrating that RD21 provides immunity to a necrotrophic pathogen.
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影响因子：
--
作者：
Greenbaum, D;Medzihradszky, KF;Bogyo, M
通讯作者：
Bogyo, M
影响因子：
7.2
作者：
PARKER, JE;SZABO, V;JONES, JDG
通讯作者：
JONES, JDG
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
56.9
作者：
Rooney, HCE;van 't Klooster, JW;de Wit, PJGM
通讯作者：
de Wit, PJGM
影响因子：
56.9
作者：
Krüger, J;Thomas, CM;Jones, JDG
通讯作者：
Jones, JDG