The Impact of Anesthetic State on Spike-Sorting Success in the Cortex: A Comparison of Ketamine and Urethane Anesthesia.
The Impact of Anesthetic State on Spike-Sorting Success in the Cortex: A Comparison of Ketamine and Urethane Anesthesia.
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DOI：
10.3389/fncir.2017.00095
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发表时间：
2017
影响因子：
3.5
通讯作者：
Linden JF
中科院分区：
文献类型：
--
作者：
Hildebrandt KJ;Sahani M;Linden JF
尖峰分选是细胞外体内电生理记录的大多数分析中必不可少的第一步。在这里，我们表明，尖峰分选的成功关键取决于协调人口活动的特点，可以不同的麻醉状态。在小鼠听觉皮层的四极记录中，氯胺酮/美托咪定（ket/med）麻醉下的棘波分选显著低于乌拉坦麻醉。令人惊讶的是，在ket/med麻醉下进行分类的困难似乎不是由于神经活动的毫秒级突发性或相邻神经元之间活动的协调性增加。相反，影响分类成功的关键因素似乎是在长时间间隔和大皮层距离上协调的群体活动量。我们建议，尖峰分选的成功是直接依赖于整体协调的活动，是最大的破坏大规模的波动，在皮质人口活动。因此，单单位记录的可靠性可能不仅在麻醉状态和麻醉/药物麻醉状态之间存在差异，而且在未麻醉动物的同步和去麻醉状态、睡眠和清醒状态或疏忽和注意状态之间也存在差异。
Spike sorting is an essential first step in most analyses of extracellular in vivo electrophysiological recordings. Here we show that spike-sorting success depends critically on characteristics of coordinated population activity that can differ between anesthetic states. In tetrode recordings from mouse auditory cortex, spike sorting was significantly less successful under ketamine/medetomidine (ket/med) than urethane anesthesia. Surprisingly, this difficulty with sorting under ket/med anesthesia did not appear to result from either greater millisecond-scale burstiness of neural activity or increased coordination of activity among neighboring neurons. Rather, the key factor affecting sorting success appeared to be the amount of coordinated population activity at long time intervals and across large cortical distances. We propose that spike-sorting success is directly dependent on overall coordination of activity, and is most disrupted by large-scale fluctuations in cortical population activity. Reliability of single-unit recording may therefore differ not only between urethane-anesthetized and ket/med-anesthetized states as demonstrated here, but also between synchronized and desynchronized states, asleep and awake states, or inattentive and attentive states in unanesthetized animals.
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DOI：
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发表时间：
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期刊：
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