Characterization of Salmonella enterica Contamination in Pork and Poultry Meat from São Paulo/Brazil: Serotypes, Genotypes and Antimicrobial Resistance Profiles.
Characterization of Salmonella enterica Contamination in Pork and Poultry Meat from São Paulo/Brazil: Serotypes, Genotypes and Antimicrobial Resistance Profiles.
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DOI：
10.3390/pathogens11030358
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发表时间：
2022-03-16
期刊：
影响因子：
--
通讯作者：
Moreno AM
中科院分区：
文献类型：
--
作者：
Gomes VTM;Moreno LZ;Silva APS;Thakur S;La Ragione RM;Mather AE;Moreno AM
沙门氏菌病是一种与全球公共卫生密切相关的人畜共患病。在这里，我们提出了在巴西圣保罗零售市场销售的猪肉和禽肉中肠道沙门氏菌污染的评估。食环署从132个街市共抽取780个肉类样本（386个禽肉样本及394个猪肉样本）。其中，57份样品（7.3%）为S阳性。肠道分离，包括32份（8.3%）禽肉和25份（6.3%）猪肉样品。S.通过扩增片段长度多态性和脉冲场凝胶电泳进一步表征肠杆菌分离株的血清分型、抗微生物剂抗性和基因分型。耐药性分析表明，猪肉分离株对大环内酯类、β-内酰胺类、四环素类、酚类和氟喹诺酮类耐药率较高，禽肉分离株对氟喹诺酮类、磺胺类、四环素类和β-内酰胺类耐药率较高。禽肉分离株经鉴定为沙门氏菌占72.4%。Heidelberg，而猪肉分离物大多为S.鼠伤寒沙门氏菌（31.7%）和沙门氏菌（31.7%）;给予（16.7%）。基因分型导致大多数集群完全由猪肉或禽肉，没有交叉污染被检测到，并根据其血清型和原产地的市场来区分分离株的趋势。对至关重要的抗菌剂的高耐药率加强了控制肉类生产链中沙门氏菌污染的重要性。
Salmonellosis is a zoonosis of major relevance to global public health. Here we present the assessment of Salmonella enterica contamination in pork and poultry meat sold at retail markets in São Paulo, Brazil. A total of 780 meat samples (386 poultry meat and 394 pork samples) were collected from 132 markets. From these, 57 samples (7.3%) were positive for S. enterica isolation, including 32 (8.3%) poultry meat and 25 (6.3%) pork samples. S. enterica isolates were further characterized for serotyping, antimicrobial resistance and genotyping by amplified fragment length polymorphism and pulsed field gel electrophoresis. Antimicrobial resistance analysis demonstrated two main profiles: pork isolates were more resistant to macrolides, β-lactams, tetracycline, phenicols, and fluoroquinolones, and poultry meat isolates presented higher resistance to fluoroquinolones, sulfonamides, tetracycline, and β-lactams. A total of 72.4% of poultry meat isolates were identified as S. Heidelberg, while most of pork isolates were S. Typhimurium (31.7%) and S. Give (16.7%). Genotyping resulted in most clusters consisting exclusively of pork or poultry meat, no cross-contamination was detected, and a tendency to differentiate isolates according to their serotypes and markets of origin. High resistance rates to critically important antimicrobials reinforce the importance of controlling Salmonella contamination in meat production chains.
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影响因子：
4.4
作者：
Lemos MPL;Saraiva MMS;Leite EL;Silva NMV;Vasconcelos PC;Giachetto PF;Freitas Neto OC;Givisiez PEN;Gebreyes WA;Oliveira CJB
通讯作者：
Oliveira CJB
影响因子：
9.4
作者：
HUNTER, PR;GASTON, MA
通讯作者：
GASTON, MA
影响因子：
2.8
作者：
Ribot, Efrain M.;Fair, M. A.;Barrett, Timothy J.
通讯作者：
Barrett, Timothy J.
DOI：
10.1016/j.ijfoodmicro.2021.109091
发表时间：
2021-02-24
影响因子：
5.4
作者：
de Melo, Adma Nadja Ferreira;Monte, Daniel F. M.;Macarisin, Dumitru
通讯作者：
Macarisin, Dumitru
影响因子：
6
作者：
Muermann, Lisandra;dos Santos, Maria Cecilia;Cardoso, Marisa
通讯作者：
Cardoso, Marisa