Differential gene expression in motor and sensory Schwann cells in the rat femoral nerve.
Differential gene expression in motor and sensory Schwann cells in the rat femoral nerve.
复制标题
DOI：
10.1002/jnr.22752
复制
发表时间：
2012-01
影响因子：
4.2
通讯作者：
Sakiyama-Elbert, Shelly E.
中科院分区：
文献类型：
--
作者：
Jesuraj, Nithya J.;Nguyen, Peter K.;Wood, Matthew D.;Moore, Amy M.;Borschel, Gregory H.;Mackinnon, Susan E.;Sakiyama-Elbert, Shelly E.
雪旺细胞（SC）的表型差异可能有助于引导周围神经损伤后轴突沿着运动或感觉特定途径再生。本研究的目的是鉴定从大鼠股神经的皮肤（感觉）和股四头肌（运动）分支收获的 SC 的表型标记，并研究扩增培养对这些运动或感觉表型标记表达模式的影响。从大鼠股神经运动和感觉分支收获的 SC 中提取 RNA，并使用 Affymetrix Gene Chips©（Rat Genome 230 v2.0 Array A）进行分析。与感觉 SC 相比，运动 SC 中上调的基因被识别出来，反之亦然，并使用定量实时聚合酶链反应 (qRT-PCR) 对一部分基因的结果进行了验证。然后通过 qRT-PCR 在 30 天的不同时间点在 SC 扩增培养物中评估“表型特异性”基因的表达水平，以确定扩增对 SC 表型的影响。与新鲜神经组织相比，运动和感觉标记物扩增培养后表型特异性基因的表达水平显着改变。这些结果表明，运动和感觉 SC 基因表达模式在体外扩增过程中均被破坏，并可能影响 SC 在移植后表达表型特异性基因的能力。
Phenotypic differences in Schwann cells (SCs) may help guide axonal regeneration down motor or sensory specific pathways following peripheral nerve injury. The goal of this study was to identify phenotypic markers for SCs harvested from the cutaneous (sensory) and quadriceps (motor) branches of the rat femoral nerve and to study the effects of expansion culture on the expression patterns of these motor or sensory phenotypic markers. RNA was extracted from SCs harvested from the motor and sensory branches of the rat femoral nerve and analyzed using Affymetrix Gene Chips© (Rat Genome 230 v2.0 Array A). Genes that were upregulated in motor SCs compared to the sensory SCs or vice versa were identified, and the results were verified for a subset of genes using quantitative real time polymerase chain reaction (qRT-PCR). The expression levels of the “phenotype-specific” genes were then evaluated in SC expansion cultures at various timepoints over 30 days by qRT-PCR to determine the effect of expansion on SC phenotype. Expression levels of the phenotype-specific genes were significantly altered after expansion culture for both the motor and sensory markers compared to fresh nerve tissue. These results indicate that both motor and sensory SC gene expression patterns are disrupted during expansion in vitro and may affect the ability of SCs to express phenotype specific genes after transplantation.
登录
查看更多内容
影响因子：
3.7
作者：
Biswas S;Reinhard J;Oakeshott J;Russell R;Srinivasan MV;Claudianos C
通讯作者：
Claudianos C
影响因子：
6.2
作者：
LEVI, ADO;EVANS, PJ;BUNGE, RP
通讯作者：
BUNGE, RP
DOI：
10.1126/science.1166562
发表时间：
2009-01-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kao SC;Wu H;Xie J;Chang CP;Ranish JA;Graef IA;Crabtree GR
通讯作者：
Crabtree GR
影响因子：
6.2
作者：
HARE, GMT;EVANS, PJ;HAY, JB
通讯作者：
HAY, JB
影响因子：
2.1
作者：
Brenner, MJ;Lowe, JB;Mohanakumar, T
通讯作者：
Mohanakumar, T