Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels.
Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels.
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DOI：
10.3389/fcell.2016.00139
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发表时间：
2016
影响因子：
5.5
通讯作者：
Miyata T
中科院分区：
文献类型：
--
作者：
Nagasaka A;Shinoda T;Kawaue T;Suzuki M;Nagayama K;Matsumoto T;Ueno N;Kawaguchi A;Miyata T
发育中的组织中的细胞产生事件在整个细胞周期中是机械动态的。在许多上皮系统中，细胞是顶基高的，细胞核和胞体采用不同的顶基位置，因为细胞核和胞体以细胞周期依赖的方式移动。这种运动在G2期是顶端的，在G1期是基部的，而有丝分裂发生在顶端表面。这些运动统称为核间迁移，这种上皮细胞被称为“假复层”。胚胎哺乳动物大脑皮质神经上皮是高度假复层上皮的良好模型，我们以前发现小鼠和雪貂之间的差异，在水平细胞密度（雪貂更大）和核/体运动（G2期间较慢，G1期间更快雪貂）。这些差异表明，神经上皮细胞改变它们的核动力学行为，以响应它们遇到的物理因素，这可能构成从小鼠到雪貂和灵长类动物向更丰富的脑细胞生产进化过渡的基础。为了解决如何小鼠和雪貂神经上皮细胞可能会在物理上不同的定量方式，我们使用原子力显微镜来确定其顶端表面的垂直刚度是更大的雪貂（杨氏模量= 1700 Pa）比小鼠（1400 Pa）。我们通过实验系统地分析了根尖表面硬度的潜在因素，以抑制肌动球蛋白或微管，并检查激光消融后根尖表面的反冲行为，并通过电子显微镜观察粘附连接。我们发现，虽然肌动球蛋白和微管是部分负责的顶端表面刚度，小鼠<雪貂的关系，即使在抑制剂的存在下，保持在顶端表面刚度。我们还发现，小鼠（720 Pa）中单个分离的神经上皮细胞的硬度实际上大于雪貂（450 Pa）。小鼠和雪貂之间的粘附连接在超微结构上相当。这些结果表明，神经上皮细胞过程的水平密集的包装是一个主要的贡献者，在雪貂的组织水平的顶端刚度增加，并建议，组织水平的机械性能可以通过平衡细胞致密化和单细胞的物理性能来实现。
Cell-producing events in developing tissues are mechanically dynamic throughout the cell cycle. In many epithelial systems, cells are apicobasally tall, with nuclei and somata that adopt different apicobasal positions because nuclei and somata move in a cell cycle–dependent manner. This movement is apical during G2 phase and basal during G1 phase, whereas mitosis occurs at the apical surface. These movements are collectively referred to as interkinetic nuclear migration, and such epithelia are called “pseudostratified.” The embryonic mammalian cerebral cortical neuroepithelium is a good model for highly pseudostratified epithelia, and we previously found differences between mice and ferrets in both horizontal cellular density (greater in ferrets) and nuclear/somal movements (slower during G2 and faster during G1 in ferrets). These differences suggest that neuroepithelial cells alter their nucleokinetic behavior in response to physical factors that they encounter, which may form the basis for evolutionary transitions toward more abundant brain-cell production from mice to ferrets and primates. To address how mouse and ferret neuroepithelia may differ physically in a quantitative manner, we used atomic force microscopy to determine that the vertical stiffness of their apical surface is greater in ferrets (Young's modulus = 1700 Pa) than in mice (1400 Pa). We systematically analyzed factors underlying the apical-surface stiffness through experiments to pharmacologically inhibit actomyosin or microtubules and to examine recoiling behaviors of the apical surface upon laser ablation and also through electron microscopy to observe adherens junction. We found that although both actomyosin and microtubules are partly responsible for the apical-surface stiffness, the mouse<ferret relationship in the apical-surface stiffness was maintained even in the presence of inhibitors. We also found that the stiffness of single, dissociated neuroepithelial cells is actually greater in mice (720 Pa) than in ferrets (450 Pa). Adherens junction was ultrastructurally comparable between mice and ferrets. These results show that the horizontally denser packing of neuroepithelial cell processes is a major contributor to the increased tissue-level apical stiffness in ferrets, and suggest that tissue-level mechanical properties may be achieved by balancing cellular densification and the physical properties of single cells.
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影响因子：
3.5
作者：
Crick, S. L.;Yin, F. C. -P.
通讯作者：
Yin, F. C. -P.
DOI：
10.1083/jcb.201509020
发表时间：
2016-02-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Katsunuma S;Honda H;Shinoda T;Ishimoto Y;Miyata T;Kiyonari H;Abe T;Nibu K;Takai Y;Togashi H
通讯作者：
Togashi H
DOI：
10.1111/dgd.12131
发表时间：
2014-05
期刊：
Development, growth & differentiation
影响因子：
--
作者：
Kawaue T;Sagou K;Kiyonari H;Ota K;Okamoto M;Shinoda T;Kawaguchi A;Miyata T
通讯作者：
Miyata T
影响因子：
1.6
作者：
HUTTER, JL;BECHHOEFER, J
通讯作者：
BECHHOEFER, J
影响因子：
112.7
作者：
Iskratsch, Thomas;Wolfenson, Haguy;Sheetz, Michael P.
通讯作者：
Sheetz, Michael P.