Improving metabolic health in obese male mice via diet and exercise restores embryo development and fetal growth.
Improving metabolic health in obese male mice via diet and exercise restores embryo development and fetal growth.
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DOI：
10.1371/journal.pone.0071459
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lane M
中科院分区：
文献类型：
--
作者：
McPherson NO;Bakos HW;Owens JA;Setchell BP;Lane M
父亲肥胖现在明确与人类和啮齿动物的胚胎和胎儿发育受损以及妊娠率降低有关或因果关系。这似乎是胚泡潜力降低的结果。这些不良的胚胎和胎儿结局是否可以通过干预措施来改善，以减少父亲的肥胖尚未确定。在这里，使用喂食高脂肪饮食（HFD）以诱导肥胖的雄性小鼠，以确定通过饮食（CD）和/或运动的干预以减少肥胖和改善代谢是否改善早期胚胎和胎儿发育。运动和在较小程度上的CD肥胖男性改善胚胎发育率，增加细胞与细胞接触的致密胚胎测量E-钙粘蛋白在运动干预，随后，增加囊胚滋养外胚层（TE），内细胞团（ICM）和上胚层细胞数量。肥胖男性的着床率和胚胎发育也通过运动得到改善。此外，对肥胖男性的所有干预措施增加了胎儿体重，单独使用CD和单独使用运动，也增加了胎儿顶臀长度。胚胎和胎儿发育的测量与父亲的血脂，胰岛素作用和血脂的测量相关，无论父亲的肥胖或干预，这表明父亲的代谢健康和随后的胚胎和胎儿发育之间的联系。这是第一项研究表明，通过饮食和运动改善肥胖男性的代谢健康可以改善胚胎和胎儿发育，这表明这种干预可能会改善后代的健康。
Paternal obesity is now clearly associated with or causal of impaired embryo and fetal development and reduced pregnancy rates in humans and rodents. This appears to be a result of reduced blastocyst potential. Whether these adverse embryo and fetal outcomes can be ameliorated by interventions to reduce paternal obesity has not been established. Here, male mice fed a high fat diet (HFD) to induce obesity were used, to determine if early embryo and fetal development is improved by interventions of diet (CD) and/or exercise to reduce adiposity and improve metabolism. Exercise and to a lesser extent CD in obese males improved embryo development rates, with increased cell to cell contacts in the compacting embryo measured by E-cadherin in exercise interventions and subsequently, increased blastocyst trophectoderm (TE), inner cell mass (ICM) and epiblast cell numbers. Implantation rates and fetal development from resulting blastocysts were also improved by exercise in obese males. Additionally, all interventions to obese males increased fetal weight, with CD alone and exercise alone, also increasing fetal crown-rump length. Measures of embryo and fetal development correlated with paternal measures of glycaemia, insulin action and serum lipids regardless of paternal adiposity or intervention, suggesting a link between paternal metabolic health and subsequent embryo and fetal development. This is the first study to show that improvements to metabolic health of obese males through diet and exercise can improve embryo and fetal development, suggesting such interventions are likely to improve offspring health.
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