Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
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DOI：
10.1083/jcb.107.5.1853
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发表时间：
1988-11
期刊：
影响因子：
--
通讯作者：
Butcher EC
中科院分区：
文献类型：
--
作者：
Streeter PR;Rouse BT;Butcher EC
组织定位或循环淋巴细胞的“归巢”部分是由专门的血管引导的，这些血管定义了淋巴细胞从血液中退出的位置。例如，在外周淋巴结、粘膜淋巴组织（Peyer’s斑块和阑尾）和慢性炎症部位，淋巴细胞通过粘附和在毛细血管后高内皮小静脉（HEV）内层的内皮细胞之间迁移而离开血液。淋巴细胞与HEV相互作用的功能分析表明，淋巴细胞可以区分不同组织中的HEV，表明HEV表达组织特异性决定因素或淋巴细胞识别的地址信号。我们最近描述了这样一种组织特异性的“血管寻址”，它是由内皮细胞选择性表达的，支持淋巴细胞外渗到粘膜组织中，这似乎是粘膜特异性淋巴细胞归巢所必需的（Streeter, p.r., e.l. Berg, b.n. Rouse, r.f. Bargatze和e.c. Butcher, 1988）。自然(Lond)。331:41 - 46)。在这里，我们记录了一种独特的HEV分化抗原的存在和组织特异性分布。该抗原由单克隆抗体MECA-79定义，在HEV周围淋巴结的管腔表面和细胞质中高水平表达。相比之下，尽管MECA-79在粘膜Peyer's斑块中染色了许多HEV，但在大多数情况下，表达仅限于这些小静脉的血管周围或管腔方面。在小肠固有层，一个支持淋巴细胞外渗的粘膜相关部位，小静脉不被MECA-79染色。最后，我们证明了MECA-79在体外阻断正常淋巴细胞和外周淋巴结特异性淋巴瘤与HEV的结合，并且在体内也抑制正常淋巴细胞归巢到外周淋巴结，而在体外或体内不显著影响淋巴细胞与Peyer贴片HEV的相互作用。因此，MECA-79定义了一种新的血管寻址蛋白，参与指导淋巴细胞归巢到周围淋巴结。
The tissue localization or "homing" of circulating lymphocytes is directed in part by specialized vessels that define sites of lymphocyte exit from the blood. In peripheral lymph nodes, mucosal lymphoid tissues (Peyer's patches and appendix), and sites of chronic inflammation, for example, lymphocytes leave the blood by adhering to and migrating between those endothelial cells lining postcapillary high endothelial venules (HEV). Functional analyses of lymphocyte interactions with HEV have shown the lymphocytes can discriminate between HEV in different tissues, indicating that HEV express tissue- specific determinants or address signals for lymphocyte recognition. We recently described such a tissue-specific "vascular addressin" that is selectively expressed by endothelial cells supporting lymphocyte extravasation into mucosal tissues and that appears to be required for mucosa-specific lymphocyte homing (Streeter, P. R., E. L. Berg, B. N. Rouse, R. F. Bargatze, and E. C. Butcher. 1988. Nature (Lond.). 331:41- 46). Here we document the existence and tissue-specific distribution of a distinct HEV differentiation antigen. Defined by monoclonal antibody MECA-79, this antigen is expressed at high levels on the lumenal surface and in the cytoplasm of HEV in peripheral lymph nodes. By contrast, although MECA-79 stains many HEV in the mucosal Peyer's patches, expression in most cases is restricted to the perivascular or ablumenal aspect of these venules. In the small intestine lamina propria, a mucosa-associated site that supports the extravasation of lymphocytes, venules do not stain with MECA-79. Finally, we demonstrate that MECA-79 blocks binding of both normal lymphocytes and a peripheral lymph node-specific lymphoma to peripheral lymph node HEV in vitro and that it also inhibits normal lymphocyte homing to peripheral lymph nodes in vivo without significantly influencing lymphocyte interactions with Peyer's patch HEV in vitro or in vivo. Thus, MECA-79 defines a novel vascular addressin involved in directing lymphocyte homing to peripheral lymph nodes.
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影响因子：
64.8
作者：
GALLATIN, WM;WEISSMAN, IL;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
7.8
作者：
Jalkanen, S;Bargatze, R F;de los Toyos, J;Butcher, E C
通讯作者：
Butcher, E C
DOI：
10.1073/pnas.83.23.9114
发表时间：
1986-12-01
影响因子：
11.1
作者：
DUIJVESTIJN, AM;SCHREIBER, AB;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
5.4
作者：
HALL, JG;HOPKINS, J;ORLANS, E
通讯作者：
ORLANS, E
影响因子：
5.4
作者：
HENDRIKS, HR;EESTERMANS, IL
通讯作者：
EESTERMANS, IL