A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity.
A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity.
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马铃薯 STRUBBELIG 受体家族成员 StLRPK1 与 StSERK3A/BAK1 结合并激活免疫力。
DOI：
10.1093/jxb/ery310
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发表时间：
2018-11-26
影响因子：
6.9
通讯作者：
Tian Z
中科院分区：
文献类型：
--
作者：
Wang H;Chen Y;Wu X;Long Z;Sun C;Wang H;Wang S;Birch PRJ;Tian Z
马铃薯STRUBBELIG受体家族蛋白StLRPK1与StSERK3A/BAK1结合，通过MAPK信号通路正向调节马铃薯和烟草的免疫。植物STRUBBELIG(亚)受体家族(SRF)基因编码富含亮氨酸的重复跨膜受体样蛋白。据报道，SRF在许多植物器官的组织形态发生中起着重要作用。在这里，我们展示了一个马铃薯SRF家族基因StLRPK1，它与植物免疫有关。StLRPK1位于细胞膜上，受马铃薯晚疫病菌体外培养滤液的强烈诱导。在稳定的转基因马铃薯中过表达StLRPK1或在烟草植株中异源表达StLRPK1可提高致病疫霉的抗病能力，而在马铃薯中进行StLRPK1的RNA干扰(RNAi)可降低抗病能力。我们发现StLRPK1与关键的共受体SERK3A/BAK1发生结构性相互作用，SERK3A/BAK1在植物免疫中起核心作用。病毒诱导的SERK3A/BAK1在表达StLRPK1的本底夜蛾中的基因沉默降低了致病疫霉的抗性，表明SERK3A/BAK1是StLRPK1介导的免疫所必需的。最后，我们证明了StLRPK1触发的晚疫病抗性依赖于丝裂原活化蛋白激酶MEK2和丝裂原活化蛋白激酶WIPK。我们提出了一个模型，其中StLRPK1与SERK3A/BAK1结合，通过MAPK级联信号通路正向调节植物对致病疫霉的免疫。这些数据为我们理解SRF在植物免疫中的作用提供了新的见解。
The potato STRUBBELIG-RECEPTOR FAMILY protein StLRPK1 associates with StSERK3A/BAK1 to positively regulate potato and Nicotiana benthamiana immunity through a MAPK signaling pathway. Plant STRUBBELIG (SUB)-RECEPTOR FAMILY (SRF) genes encode putative leucine-rich repeat transmembrane receptor-like kinases. SRFs have been reported to play essential roles in tissue morphogenesis in many plant organs. Here, we show that a potato SRF family gene, StLRPK1, is involved in plant immunity. StLRPK1 is located at the cell plasma membrane and is strongly induced by culture filtrate from in vitro growth of the late blight pathogen Phytophthora infestans. Overexpression of StLRPK1 in stable transgenic potato or ectopic expression in Nicotiana benthamiana plants enhances P. infestans disease resistance, whereas RNA interference (RNAi) of StLRPK1 in potato decreases disease resistance. We found that StLRPK1 constitutively interacts with a pivotal co-receptor, SERK3A/BAK1, which plays a central role in plant immunity. Virus-induced gene silencing of SERK3A/BAK1 in N. benthamiana lines expressing StLRPK1 attenuated P. infestans resistance, indicating that SERK3A/BAK1 is required for StLRPK1-mediated immunity. Finally, we show that StLRPK1-triggered late blight resistance depends on the mitogen-activated protein kinase kinase MEK2 and mitogen-activated protein kinase WIPK. We propose a model in which StLRPK1 associates with SERK3A/BAK1 to positively regulate plant immunity to P. infestans through a MAPK cascade. These data provide new insights into our understanding of SRF function in plant immunity.
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影响因子：
18
作者：
Du, Juan;Verzaux, Estelle;Vleeshouwers, Vivianne G. A. A.
通讯作者：
Vleeshouwers, Vivianne G. A. A.
影响因子：
3.7
作者：
Chaparro-Garcia A;Wilkinson RC;Gimenez-Ibanez S;Findlay K;Coffey MD;Zipfel C;Rathjen JP;Kamoun S;Schornack S
通讯作者：
Schornack S
U-box E3 泛素连接酶 PUB17 在细胞核中发挥作用，促进致病疫霉触发的特定免疫途径。
影响因子：
6.9
作者：
He Q;McLellan H;Boevink PC;Sadanandom A;Xie C;Birch PR;Tian Z
通讯作者：
Tian Z
DOI：
10.1111/j.1365-313x.2011.04518.x
发表时间：
2011-04
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Gish LA;Clark SE
通讯作者：
Clark SE
DOI：
10.1073/pnas.0508882103
发表时间：
2006-07-18
影响因子：
11.1
作者：
Kaku, Hanae;Nishizawa, Yoko;Shibuya, Naoto
通讯作者：
Shibuya, Naoto