In vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59.
In vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59.
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DOI：
10.1016/0042-6822(87)90293-5
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发表时间：
1987-04
期刊：
影响因子：
3.7
通讯作者：
Weiss SR
中科院分区：
文献类型：
--
作者：
Budzilowicz CJ;Weiss SR
测定了冠状病毒小鼠肝炎病毒（MHV）A59株非结构基因5的全序列。该序列含有两个潜在的开放阅读框，其重叠5个核苷酸。从序列预测的推定蛋白质产物是基本的13，000-Da多肽和含有异常长的疏水氨基末端的9600-Da多肽。在pGEM载体中从含有每个开放阅读框的DNA体外转录的RNA指导体外合成由序列预测大小的多肽。含有两个开放阅读框的RNA转录物主要指导对应于下游开放阅读框的多肽的合成。这些数据表明，MHV-A59信使RNA 5可能编码两种蛋白质，并可能优先从内部AUG起始密码子翻译。
The complete nucleotide sequence of nonstructural gene 5 of coronavirus mouse hepatitis virus (MHV) strain A59 has been determined. This sequence contains two potential open reading frames which overlap by five nucleotides. The putative protein products predicted from the sequence are a basic 13,000-Da polypeptide and a 9600-Da polypeptide containing an unusually long hydrophobic amino terminus. RNAs transcribed in vitro from DNAs containing each of the open reading frames in pGEM vectors direct the synthesis in vitro of polypeptides of the sizes predicted by the sequence. An RNA transcript containing both of the open reading frames directs the synthesis primarily of the polypeptide corresponding to the downstream open reading frame. These data suggest that MHV-A59 messenger RNA 5 potentially encodes two proteins and may be preferentially translated from an internal AUG initiation codon.
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影响因子：
5.4
作者：
BRAYTON, PR;LAI, MMC;STOHLMAN, SA
通讯作者：
STOHLMAN, SA
影响因子：
5.4
作者：
HIEBERT, SW;PATERSON, RG;LAMB, RA
通讯作者：
LAMB, RA
DOI：
10.1073/pnas.82.1.48
发表时间：
1985-01-01
影响因子：
11.1
作者：
ERNST, H;SHATKIN, AJ
通讯作者：
SHATKIN, AJ
影响因子：
5.4
作者：
JACOBS, L;SPAAN, WJM;VANDERZEIJST, BAM
通讯作者：
VANDERZEIJST, BAM
影响因子：
5.4
作者：
LEIBOWITZ, JL;WEISS, SR;BOND, CW
通讯作者：
BOND, CW