Reduction of NADPH oxidase 4 in adipocytes contributes to the anti-obesity effect of dihydroartemisinin.
Reduction of NADPH oxidase 4 in adipocytes contributes to the anti-obesity effect of dihydroartemisinin.
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DOI：
10.1016/j.heliyon.2023.e14028
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发表时间：
2023-03
期刊：
影响因子：
4
通讯作者：
Jia, Zhanjun
中科院分区：
文献类型：
--
作者：
Hua, Hu;Wu, Mengqiu;Wu, Tong;Ji, Yong;Jin, Lv;Du, Yang;Zhang, Yue;Huang, Songming;Zhang, Aihua;Ding, Guixia;Liu, Qianqi;Jia, Zhanjun
近年来，人们发现青蒿素衍生物具有抗肥胖作用，但其机制仍存在争议。在这项研究中，肥胖小鼠长期服用DHA显著降低了体重，改善了葡萄糖代谢。然而，短期DHA治疗并没有影响肥胖小鼠的糖代谢，这表明DHA治疗小鼠糖代谢的改善可能是继发于体重减轻。与之前的报道一致，我们观察到DHA抑制脂肪细胞的分化。机制上，DHA可显著降低小鼠白色脂肪组织（WAT）中NADPH氧化酶4 （NOX4）的表达和分化脂肪细胞，使用NOX4 siRNA或NOX4抑制剂GKT137831可显著减弱脂肪细胞分化。NOX4的过表达部分逆转了DHA对前脂肪细胞成脂分化的抑制作用。此外，靶向蛋白质组学分析表明，DHA改善了代谢途径的异常。综上所述，DHA可能通过抑制NOX4的表达抑制脂肪细胞分化和脂肪细胞中的脂质积累，从而显著降低肥胖小鼠的脂肪质量，改善糖代谢。DHA可能通过改善肥胖改善肥胖小鼠的葡萄糖代谢。DHA减少白色脂肪组织中的脂质积累和分化脂肪细胞。DHA抑制脂肪细胞分化可能是通过抑制NOX4的表达。DHA改善了代谢途径的异常。
Artemisinin derivatives have been found to have anti-obesity effects recently, but the mechanism is still controversial. Herein, long-term DHA treatment in obese mice significantly reduced the body weight and improved glucose metabolism. However, short-term DHA treatment did not affect glucose metabolism in obese mice, suggesting that the improved glucose metabolism in mice with DHA treatment could be secondary to body weight reduction. Consistent with previous reports, we observed that DHA inhibited the differentiation of adipocytes. Mechanistically, DHA significantly reduced the expression of NADPH oxidase 4 (NOX4) in white adipose tissue (WAT) of mice and differentiated adipocytes, and using NOX4 siRNA or the NOX4 inhibitor GKT137831 significantly attenuated adipocyte differentiation. Over-expression of NOX4 partially reversed the inhibition effect of DHA on adipogenic differentiation of preadipocytes. In addition, targeted proteomics analysis showed that DHA improved the abnormality of metabolic pathways. In conclusion, DHA significantly reduced fat mass and improved glucose metabolism in obese mice, possibly by inhibiting NOX4 expression to suppress adipocyte differentiation and lipid accumulation in adipocytes. DHA improved glucose metabolism in obese mice possibly by ameliorating obesity. DHA attenuated lipid accumulation in white adipose tissues and differentiated adipocytes. DHA inhibited adipocyte differentiation possibly by inhibiting NOX4 expression. DHA improved the abnormality of metabolic pathways.
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影响因子：
5.8
作者：
Nugent JL;Singh A;Wirth KM;Oppler SH;Hocum Stone L;Janecek JL;Sheka AC;Kizy S;Moore MEG;Staley C;Hering BJ;Ramachandran S;Ikramuddin S;Graham ML
通讯作者：
Graham ML
MiR-146b 是人内脏前脂肪细胞增殖和分化的调节因子，其表达在人类肥胖中发生改变
影响因子：
4.1
作者：
Chen, Ling;Dai, Yong-Mei;Guo, Xi-Rong
通讯作者：
Guo, Xi-Rong
影响因子：
2.9
作者：
Chen, Joanna Y.;Singh, Simar;Lowe, Michael R.
通讯作者：
Lowe, Michael R.
DOI：
10.1016/j.bbamcr.2007.03.003
发表时间：
2007-07-01
影响因子：
5.1
作者：
Mouche, Sarah;Ben Mkaddem, Sanae;Szanto, Ildiko
通讯作者：
Szanto, Ildiko
影响因子：
5.9
作者：
通讯作者：
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