Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis.
Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis.
复制标题
单轴固定的力学边界条件诱导细胞在胶原基质中排列，诱导成骨。
DOI：
10.1038/s41598-021-88505-z
复制
发表时间：
2021-04-27
影响因子：
4.6
通讯作者：
Adachi T
中科院分区：
文献类型：
--
作者：
Kim J;Ishikawa K;Sunaga J;Adachi T
由成骨细胞分化而来的骨细胞在调节骨重建过程中起着重要的力学传感器作用。虽然已知骨小梁上沿着排列良好的骨细胞网络（具有垂直于骨小梁表面的细长细胞突起）有助于细胞对机械刺激的感知和骨基质中的细胞内通讯，但骨细胞网络形成的机制仍不清楚。在这里，我们开发了一种新的体外胶原蛋白基质系统施加单轴固定的机械边界条件，小鼠成骨样MC 3 T3-E1细胞进行传代培养，引起细胞排列沿着单轴边界条件。使用肌球蛋白II抑制剂，blebbistatin，我们表明，通过收缩肌动蛋白纤维的细胞内张力的影响下，等距矩阵条件沿着单轴固定的机械边界条件的细胞排列。此外，细胞在胶原基质内积极迁移，并促进成骨细胞和骨细胞基因的表达，其取向沿着单轴固定的边界条件对齐。总的来说，我们的结果表明，单轴固定力学边界条件下成骨细胞的细胞内张力是决定骨基质内骨细胞排列的因素之一。
Osteocytes differentiated from osteoblasts play significant roles as mechanosensors in modulating the bone remodeling process. While the well-aligned osteocyte network along the trabeculae with slender cell processes perpendicular to the trabeculae surface is known to facilitate the sensing of mechanical stimuli by cells and the intracellular communication in the bone matrix, the mechanisms underlying osteocyte network formation remains unclear. Here, we developed a novel in vitro collagen matrix system exerting a uniaxially-fixed mechanical boundary condition on which mouse osteoblast-like MC3T3-E1 cells were subcultured, evoking cellular alignment along the uniaxial boundary condition. Using a myosin II inhibitor, blebbistatin, we showed that the intracellular tension via contraction of actin fibers contributed to the cellular alignment under the influence of isometric matrix condition along the uniaxially-fixed mechanical boundary condition. Furthermore, the cells actively migrated inside the collagen matrix and promoted the expression of osteoblast and osteocyte genes with their orientations aligned along the uniaxially-fixed boundary condition. Collectively, our results suggest that the intracellular tension of osteoblasts under a uniaxially-fixed mechanical boundary condition is one of the factors that determines the osteocyte alignment inside the bone matrix.
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DOI：
10.1016/j.matbio.2016.02.003
发表时间：
2016-05
期刊：
Matrix biology : journal of the International Society for Matrix Biology
影响因子：
--
作者：
Chaudhary SC;Kuzynski M;Bottini M;Beniash E;Dokland T;Mobley CG;Yadav MC;Poliard A;Kellermann O;Millán JL;Napierala D
通讯作者：
Napierala D
影响因子：
3
作者：
Kerschnitzki, Michael;Wagermaier, Wolfgang;Fratzl, Peter
通讯作者：
Fratzl, Peter
循环应变频率在体外调节血管平滑肌细胞排列中的作用。
影响因子：
3.4
作者：
Liu, Bo;Qu, Ming-Juan;Jiang, Zong-Lai
通讯作者：
Jiang, Zong-Lai
影响因子：
3.3
作者：
Hirose, Satoshi;Li, Minqi;Amizuka, Norio
通讯作者：
Amizuka, Norio
影响因子：
18.2
作者：
Robling AG;Bonewald LF
通讯作者：
Bonewald LF