A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs.
A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs.
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DOI：
10.3389/fvets.2022.902497
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
猪胸膜肺炎放线杆菌(APP)是引起养猪业重大经济损失的猪传染性胸膜肺炎(PCP)的病原。目前，接种疫苗仍然是预防该病的常用策略。这种疾病的商业疫苗，包括灭活菌素和亚单位疫苗，都有临床局限性，如副作用和低交叉保护性。在本研究中，我们研制了一种组合疫苗(Bac-subA)，该疫苗由1株血清型灭活菌和3种重组原毒素(rApxIA、rApxIIA和rApxIIIA)组成。在小鼠感染模型中，评估了它的副作用、免疫保护和交叉保护，并与商业亚单位疫苗和商业三价疫苗进行了比较。结果表明，Bac-sub疫苗无明显毒副作用，免疫后诱导的Apx毒素特异性免疫球蛋白Ig G、Ig G1和Ig G2a水平均高于市售疫苗。在与强毒株1、5和7进行攻击后，BAC-SUB疫苗提供了比市售疫苗更好的保护(分别为91.76%、100%和100%)。与接种其他两种疫苗的小鼠相比，接种Bac亚疫苗的小鼠的肺部细菌载量(LBL)要低得多，肺部病变也较轻。在猪身上进一步评估了Bac-sub疫苗的保护效果，结果表明，免疫后接种疫苗的猪的临床症状和肺部病变明显轻于未接种疫苗的猪。综上所述，Bac-Sub是一种安全有效的疫苗，对小鼠和猪的胸膜肺炎弧菌感染都有很高的保护作用。
Actinobacillus pleuropneumoniae (APP) is the etiological agent of porcine contagious pleuropneumonia (PCP) that causes great economic losses in the swine industry. Currently, vaccination is still a commonly used strategy for the prevention of the disease. Commercially available vaccines of this disease, including inactivated bacterins and subunit vaccines, have clinical limitations such as side effects and low cross-protection. In this study, a combinatorial vaccine (Bac-sub) was developed, which contained inactivated bacterial cells of a serovar 1 strain and three recombinant protoxins (rApxIA, rApxIIA, and rApxIIIA). Its side effects, immune protection, and cross-protection were evaluated and compared with a commercial subunit vaccine and a commercial trivalent bacterin in a mouse infection model. The results revealed that the Bac-sub vaccine showed no obvious side effects, and induced higher levels of Apx toxin-specific IgG, IgG1, and IgG2a than the commercial vaccines after booster. After a challenge with virulent strains of serovars 1, 5, and 7, the Bac-sub vaccine provided greater protection (91.76%, 100%, and 100%, respectively) than commercial vaccines. Much lower lung bacterial loads (LBLs) and milder lung lesions were observed in the Bac-sub-vaccinated mice than in those vaccinated with the other two vaccines. The protective efficacy of the Bac-sub vaccine was further evaluated in pigs, which showed that vaccinated pigs displayed significantly milder clinical symptoms and lung lesions than the unvaccinated pigs after the challenge. Taken together, Bac-sub is a safe and effective vaccine that could provide high protection against A. pleuropneumoniae infection in both mice and pigs.
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影响因子：
9.4
作者：
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
通讯作者：
Langford PR
影响因子：
2.6
作者：
Xie F;Li G;Zhou L;Zhang Y;Cui N;Liu S;Wang C
通讯作者：
Wang C
影响因子：
3.3
作者：
Vanni, Michele;Merenda, Marianna;Intorre, Luigi
通讯作者：
Intorre, Luigi
影响因子：
1.1
作者：
Tumamao, JQ;Bowles, RE;Blackall, PJ
通讯作者：
Blackall, PJ
影响因子：
3.2
作者：
Hennig-Pauka I;Hartmann M;Merkel J;Kreienbrock L
通讯作者：
Kreienbrock L