Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) employing mitochondrial COI gene sequence data.
Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) employing mitochondrial COI gene sequence data.
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DOI：
10.1186/1756-3305-5-248
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发表时间：
2012-11-06
影响因子：
3.2
通讯作者：
Maldonado A Jr
中科院分区：
文献类型：
--
作者：
Monte TC;Simões RO;Oliveira AP;Novaes CF;Thiengo SC;Silva AJ;Estrela PC;Maldonado A Jr
大鼠肺线虫广东管圆线虫可引起人类嗜酸性脑膜脑炎。这种线虫的主要最终宿主是啮齿动物，它的中间宿主是蜗牛。这种寄生虫最初在中国被描述，目前分布在几个太平洋岛屿、亚洲、澳大利亚、非洲、一些加勒比岛屿，最近在美洲。本文利用线粒体细胞色素c氧化酶亚基I （COI）基因序列，报道了来自巴西不同地理位置的广东南方古猿分离株的遗传变异。广东依蚊的分离株来自巴西不同的地理位置。提取基因组dna，通过聚合酶反应扩增，纯化并测序。测定了COI基因的部分序列，以评估它们的系统发育关系。广东棘球绦虫的序列呈单系分布。基于先前研究的8个不同的单倍型（ac1、ac2、ac3、ac4、ac5、ac6、ac7和ac8），我们确定了一个独特的进化支，包括来自巴西和亚洲的所有广东南方南方古猿分离株。有趣的是，巴西单倍型ac5与来自日本的分离株聚集在一起，而来自巴西里约热内卢、<s:1>圣保罗、帕尔帕<e:1>和伯南布哥州的巴西单倍型ac8形成了一个独特的进化支。巴西单倍型与中国单倍型ac6和日本单倍型ac7亲缘关系密切，我们将其命名为ac9。在巴西分离株中观察到的遗传变异支持了一种假设，即在巴西出现的广东单胞疟原虫可能是多次引入携带寄生虫的老鼠的结果，这些老鼠是在欧洲殖民时期由于与非洲和亚洲的活跃贸易而通过船只运输的。中间寄主黄斑弓形虫的迅速传播似乎也促成了该寄生虫在巴西不同地区的扩散和最终寄主的感染。
The rat lungworm Angiostrongylus cantonensis can cause eosinophilic meningoencephalitis in humans. This nematode’s main definitive hosts are rodents and its intermediate hosts are snails. This parasite was first described in China and currently is dispersed across several Pacific islands, Asia, Australia, Africa, some Caribbean islands and most recently in the Americas. Here, we report the genetic variability among A. cantonensis isolates from different geographical locations in Brazil using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. The isolates of A. cantonensis were obtained from distinct geographical locations of Brazil. Genomic DNAs were extracted, amplified by polymerase reaction, purified and sequenced. A partial sequence of COI gene was determined to assess their phylogenetic relationship. The sequences of A. cantonensis were monophyletic. We identified a distinct clade that included all isolates of A. cantonensis from Brazil and Asia based on eight distinct haplotypes (ac1, ac2, ac3, ac4, ac5, ac6, ac7 and ac8) from a previous study. Interestingly, the Brazilian haplotype ac5 is clustered with isolates from Japan, and the Brazilian haplotype ac8 from Rio de Janeiro, São Paulo, Pará and Pernambuco states formed a distinct clade. There is a divergent Brazilian haplotype, which we named ac9, closely related to Chinese haplotype ac6 and Japanese haplotype ac7. The genetic variation observed among Brazilian isolates supports the hypothesis that the appearance of A. cantonensis in Brazil is likely a result of multiple introductions of parasite-carrying rats, transported on ships due to active commerce with Africa and Asia during the European colonization period. The rapid spread of the intermediate host, Achatina fulica, also seems to have contributed to the dispersion of this parasite and the infection of the definitive host in different Brazilian regions.
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影响因子：
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作者：
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