Highly differentiated cellular and circuit properties of infralimbic pyramidal neurons projecting to the periaqueductal gray and amygdala.
Highly differentiated cellular and circuit properties of infralimbic pyramidal neurons projecting to the periaqueductal gray and amygdala.
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DOI：
10.3389/fncel.2015.00161
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发表时间：
2015
影响因子：
5.3
通讯作者：
Sheets PL
中科院分区：
文献类型：
--
作者：
Ferreira AN;Yousuf H;Dalton S;Sheets PL
边缘下皮层（inforimbic cortex，IL）是一个参与恐惧和情绪处理的互联网络中的关键节点。IL神经元接收、过滤和调制传入信号的细胞和电路水平机制仍然知之甚少，它们将这些信号投射到这个复杂网络中的不同下游节点。使用小鼠作为我们的模型，我们应用解剖标记策略，脑切片电生理学，并通过激光扫描光刺激（glu-LSPS）的笼状谷氨酸的局灶性激活的定量神经生理学分析的投影定义的神经元在IL。采用逆行示踪法，在中脑导水管周围灰质（PAG）和基底外侧杏仁核（BLA）内注射示踪剂，观察IL内皮质PAG（CP）和皮质BLA（CA）神经元。CP神经元仅在IL的第5层（L5）中发现，而CA神经元在整个IL的第2、3和5层中检测到。我们还确定了一小部分IL神经元投射到PAG和BLA。我们发现，L5 CP神经元有一个更广泛的树突状结构相比，L5 CA神经元。对急性脑片逆行标记神经元进行的神经生理记录表明，IL中的CP和CA神经元可大致分为两类：神经元共振器和非共振器。第2层CA神经元是唯一一类完全不共振的神经元。CP，CA，和CP/CA神经元在IL的第3层和第5层的谐振器和非谐振器的异质群体组成的，表明投射目标是不是一个排他性的预测内在生理。电路映射使用glu-LSPS显示，强度和组织的局部兴奋性和抑制性输入更强的CP相比，CA神经元在IL。总之，我们的研究结果建立了一个组织方案，将细胞神经生理学与IL中定义的神经元亚类的微电路参数联系起来，这些微电路参数向参与恐惧行为的皮质下结构发送下行命令。
The infralimbic (IL) cortex is a key node in an inter-connected network involved in fear and emotion processing. The cellular and circuit-level mechanisms whereby IL neurons receive, filter, and modulate incoming signals they project onward to diverse downstream nodes in this complex network remain poorly understood. Using the mouse as our model, we applied anatomical labeling strategies, brain slice electrophysiology, and focal activation of caged glutamate via laser scanning photostimulation (glu-LSPS) for quantitative neurophysiological analysis of projectionally defined neurons in IL. Injection of retrograde tracers into the periaqueductal gray (PAG) and basolateral amygdala (BLA) was used to identify cortico-PAG (CP) and cortico-BLA (CA) neurons in IL. CP neurons were found exclusively in layer 5 (L5) of IL whereas CA neurons were detected throughout layer 2, 3, and 5 of IL. We also identified a small percentage of IL neurons that project to both the PAG and the BLA. We found that L5 CP neurons have a more extensive dendritic structure compared to L5 CA neurons. Neurophysiological recordings performed on retrogradely labeled neurons in acute brain slice showed that CP and CA neurons in IL could be broadly classified in two groups: neuronal resonators and non-resonators. Layer 2 CA neurons were the only class that was exclusively non-resonating. CP, CA, and CP/CA neurons in layers 3 and 5 of IL consisted of heterogeneous populations of resonators and non-resonators showing that projection target is not an exclusive predictor of intrinsic physiology. Circuit mapping using glu-LSPS revealed that the strength and organization of local excitatory and inhibitory inputs were stronger to CP compared to CA neurons in IL. Together, our results establish an organizational scheme linking cellular neurophysiology with microcircuit parameters of defined neuronal subclasses in IL that send descending commands to subcortical structures involved in fear behavior.
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影响因子：
2.5
作者：
Hattox, Alexis M.;Nelson, Sacha B.
通讯作者：
Nelson, Sacha B.
影响因子：
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Shepherd, Gordon M. G.
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作者：
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