Field evaluation of DNA amplification of human filarial and malaria parasites using mosquito excreta/feces
Field evaluation of DNA amplification of human filarial and malaria parasites using mosquito excreta/feces
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使用蚊子排泄物/粪便对人类丝虫和疟原虫的 DNA 扩增进行现场评估
DOI：
10.1101/2019.12.23.880740
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Minetti C
中科院分区：
文献类型：
--
作者：
Minetti C
背景我们最近开发了一种基于超疏水锥的方法，用于收集蚊子排泄物/粪便（E/F），用于媒介传播寄生虫的分子异种监测，与传统方法相比，该方法具有更高的通量。为了测试其现场适用性，我们使用该平台在加纳的两个村庄检测丝虫和疟疾寄生虫的存在，并将结果与蚊子尸体和人类血液中的检测结果进行比较。（班氏吴策线虫、恶性疟原虫和持久曼氏曼氏菌），从该国萨凡纳地区两个村庄的同一户人家收集的蚊子尸体和人血。我们成功地从室内休眠的蚊子E/F中检测到寄生虫DNA，包括W。班氏综合征的社区流行率很低（2.5-3.8%）。在E/F样品的检测是一致的昆虫整体尸体和人的检测，和实验室测试表明，蚊子尸体交叉污染的风险与阳性排泄物时，昆虫一起举行的device.ConclusionsOur的方法收集和测试蚊子E/F成功地检测到各种寄生虫在不同的流行率在人群中的现场条件下，包括病原体（M。perstans），它不是由蚊子传播的。该方法显示了进一步发展和适用于早期检测和监测人类血液中携带的各种病原体的前景。作者摘要使用蚊子作为“飞行注射器”的寄生虫或病毒的分子异种监测是一种有前途的和非侵入性的工具，用于早期检测和监测各种病原体，特别是在低流行率环境中，但是需要有成本效益和更高吞吐量的替代方案。我们最近开发了一种基于收集蚊子排泄物/粪便（E/F）的新方法，该方法使用超疏水圆锥体将样品引导到收集杯底部的管中。我们测试了这种方法的能力，以检测淋巴丝虫病，疟疾，曼森氏菌病从家庭在加纳的两个流行的农村社区的存在，并将其从相应的家庭的血液和蚊子尸体样本的分子检测。蚊体E/F中寄生虫DNA的检测对三种病原体均成功，且与昆虫全尸中的检测结果一致。鉴于成功检测到不通过蚊子传播的持久曼氏菌，我们的工具显示出作为早期和非侵入性检测和监测人类血液中各种病原体的替代方法的希望，包括那些不严格蚊子传播的，在流行环境中。
BackgroundWe recently developed a superhydrophobic cone-based method for the collection of mosquito excreta/feces (E/F) for the molecular xenomonitoring of vector-borne parasites showing higher throughput compared to the traditional approach. To test its field applicability, we used this platform to detect the presence of filarial and malaria parasites in two villages of Ghana and compared results to those for detection in mosquito carcasses and human blood.Methodology and principal findingsWe compared the molecular detection of three parasites (Wuchereria bancrofti, Plasmodium falciparumandMansonella perstans) in mosquito E/F, mosquito carcasses and human blood collected from the same households in two villages in the Savannah Region of the country. We successfully detected the parasite DNA in mosquito E/F from indoor resting mosquitoes, includingW. bancroftiwhich had a very low community prevalence (2.5-3.8%). Detection in the E/F samples was concordant with detection in insect whole carcasses and people, and laboratory tests showed that the risk of mosquito carcass cross-contamination with positive excreta when insects are held together in the device is minimal.ConclusionsOur approach to collect and test mosquito E/F successfully detected a variety of parasites at varying prevalence in the human population under field conditions, including a pathogen (M. perstans) which is not transmitted by mosquitoes. The method shows promise for further development and applicability for the early detection and surveillance of a variety of pathogens carried in human blood.Author summaryMolecular xenomonitoring of parasites or viruses using mosquitoes as “flying syringes” is a promising and non-invasive tool for early detection and surveillance of various pathogens, particularly in low prevalence settings, but there is a need for cost-effective and higher throughput alternatives. We recently developed a novel approach based on the collection of mosquito excreta/feces (E/F) using a superhydrophobic cone that directs the sample into a tube at the bottom of the collection cup. We tested this method’s ability to detect the presence of lymphatic filariasis, malaria, and mansonellosis from households in two endemic rural communities of Ghana and compared it to the molecular detection from blood and mosquito carcass samples from corresponding households. The detection of parasite DNA in mosquito E/F was successful for all three pathogens, and it showed good concordance with the detection in the insects’ whole carcasses. Given the successful detection ofMansonella perstans, which is not transmitted by mosquitoes, our tool shows promise for use as an alternative method for the early and non-invasive detection and surveillance of various pathogens in human blood, including those not strictly mosquito-borne, in endemic settings.
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DOI：
--
发表时间：
2005
期刊：
影响因子：
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O. Agbolade;D. Akinboye
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