Modification of microfluidic paper-based devices with silica nanoparticles.
Modification of microfluidic paper-based devices with silica nanoparticles.
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DOI：
10.1039/c4an01147c
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发表时间：
2014-11-07
期刊：
影响因子：
--
通讯作者：
Garcia CD
中科院分区：
文献类型：
--
作者：
Evans E;Gabriel EF;Benavidez TE;Tomazelli Coltro WK;Garcia CD
本文介绍了一种二氧化硅纳米颗粒修饰的微流控纸基分析装置（μPAD），该装置具有改善的颜色强度和均匀性，可用于三种具有临床意义的酶促反应（乳酸，葡萄糖和谷氨酸）。μPADs采用Whatman 1级滤纸和CO2激光雕刻机制作。然后将经3-氨基丙基三乙氧基硅烷（APTES）修饰的二氧化硅纳米颗粒添加到纸装置中，以促进所选酶的吸附，并防止在比色测量中产生颜色梯度的洗涤效应。这里我们展示了化合物的三种不同的酶测定法。根据本文所描述的结果，二氧化硅纳米颗粒的添加在颜色强度和均匀性方面产生了显著的改善。所得μ pad可在临床相关浓度范围内检测三种分析物，乳酸、葡萄糖和谷氨酸的检出限（LOD）分别为0.63 mM、0.50 mM和0.25 mM。一个分析应用的例子已经证明了在人工尿液中所有三种分析物的半定量检测。结果表明，二氧化硅纳米颗粒在μ pad上进行比色生物测定时具有避免洗涤效应、改善颜色均匀性和强度的潜力。
This paper describes a silica nanoparticle-modified microfluidic paper-based analytical device (μPAD) with improved color intensity and uniformity for three different enzymatic reactions with clinical relevance (lactate, glucose, and glutamate). The μPADs were produced on Whatman grade 1 filter paper and using a CO2 laser engraver. Silica nanoparticles modified with 3-aminopropyltriethoxysilane (APTES) were then added to the paper devices to facilitate the adsorption of selected enzymes and prevent the washing away effect that creates color gradients in the colorimetric measurements. Here we show three different enzymatic assays for compounds. According to the results herein described, the addition of silica nanoparticles yielded to significant improvements in color intensity and uniformity. The resulting μPADs allowed for the detection of the three analytes in clinically-relevant concentration ranges with limits of detection (LOD) of 0.63 mM, 0.50 mM, and 0.25 mM for lactate, glucose, and glutamate, respectively. An example of an analytical application has been demonstrated for the semi-quantitative detection of all three analytes in artificial urine. The results demonstrate the potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs.
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影响因子：
6.2
作者：
Bhakta SA;Borba R;Taba M Jr;Garcia CD;Carrilho E
通讯作者：
Carrilho E
DOI：
10.3390/s120302456
发表时间：
2012
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Ibupoto ZH;Shah SM;Khun K;Willander M
通讯作者：
Willander M
DOI：
10.3724/sp.j.1145.2013.01069
发表时间：
2013-12-25
影响因子：
--
作者：
Alnokkari, Afraa;Ataie, Mounir;Alasaf, Zaid
通讯作者：
Alasaf, Zaid
影响因子：
6.2
作者：
Dungchai, Wijitar;Chailapakul, Orawon;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
3.9
作者：
Aissaoui, Nesrine;Bergaoui, Latifa;Boujday, Souhir
通讯作者：
Boujday, Souhir