Regulation of Drosophila eye development by the transcription factor Sine oculis.
Regulation of Drosophila eye development by the transcription factor Sine oculis.
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DOI：
10.1371/journal.pone.0089695
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Mardon G
中科院分区：
文献类型：
--
作者：
Jusiak B;Karandikar UC;Kwak SJ;Wang F;Wang H;Chen R;Mardon G
Sine oculis（SIX）家族的同源结构域转录因子在整个后生动物中指导多个调节过程。Sine oculis（So）首先在果蝇Drosophila melanogaster中被表征，在果蝇中，它对于眼睛发育、调节细胞存活、增殖和分化是必要的和足够的。尽管它在发展中的关键作用，只有少数直接目标的SO已被描述过。在目前的研究中，我们的目标是扩大我们的知识，SO介导的转录调控在发展中的果蝇眼睛使用ChIP-seq映射整个基因组中的SO结合区域。我们发现了7，566个So富集区域（峰），估计映射到5，952个基因。使用So ChIP-seq峰集和响应于so的损失或获得而差异调节的基因之间的重叠，我们鉴定了So的推定直接靶标。我们发现，所以结合富集基因以前不知道是由所以，包括编码细胞连接蛋白和信号通路组件的基因。此外，我们分析了一个子集的SO绑定的新基因的眼睛，并找到8个基因，以前未表征的眼睛表型，并可能是新的直接目标，所以。我们的研究提出了一个大大扩展的候选So目标列表，并作为未来研究So介导的眼部基因调控的基础。
Homeodomain transcription factors of the Sine oculis (SIX) family direct multiple regulatory processes throughout the metazoans. Sine oculis (So) was first characterized in the fruit fly Drosophila melanogaster, where it is both necessary and sufficient for eye development, regulating cell survival, proliferation, and differentiation. Despite its key role in development, only a few direct targets of So have been described previously. In the current study, we aim to expand our knowledge of So-mediated transcriptional regulation in the developing Drosophila eye using ChIP-seq to map So binding regions throughout the genome. We find 7,566 So enriched regions (peaks), estimated to map to 5,952 genes. Using overlap between the So ChIP-seq peak set and genes that are differentially regulated in response to loss or gain of so, we identify putative direct targets of So. We find So binding enrichment in genes not previously known to be regulated by So, including genes that encode cell junction proteins and signaling pathway components. In addition, we analyze a subset of So-bound novel genes in the eye, and find eight genes that have previously uncharacterized eye phenotypes and may be novel direct targets of So. Our study presents a greatly expanded list of candidate So targets and serves as basis for future studies of So-mediated gene regulation in the eye.
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影响因子：
64.8
作者：
BRUNNER, D;DUCKER, K;KLAMBT, C
通讯作者：
KLAMBT, C
影响因子：
64.8
作者：
FARKAS, G;GAUSZ, J;KARCH, F
通讯作者：
KARCH, F
影响因子：
3.3
作者：
Cruz, Cristina;Glavic, Alvaro;de Celis, Jose F.
通讯作者：
de Celis, Jose F.
影响因子：
2.7
作者：
Curtiss, Jennifer;Burnett, Micheal;Mlodzik, Marek
通讯作者：
Mlodzik, Marek
影响因子：
2.7
作者：
Brown, Katherine E.;Baonza, Antonio;Freeman, Matthew
通讯作者：
Freeman, Matthew