Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor β-猫眼课题宝

水貂肺上皮细胞抗转化生长因子β突变体的分离和鉴定

DOI：

10.1002/jcp.1041300102

发表时间：

1987

期刊：

Journal of Cellular Physiology

影响因子：

5.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Michael Chinkers

水貂肺上皮细胞对转化生长因子β （TGF‐β）的生长抑制具有抗性，通过化学诱变和在富含TGF‐β的血小板提取物存在下生长。几个抗性克隆被分离出来，其中至少一个在没有TGF - β的情况下生长时稳定地保持对TGF - β的抗性。该克隆细胞在形态和生长特性上与亲本细胞相似。然而，与亲本细胞不同，耐药细胞对TGF - β没有表现出以下任何反应：(1)抑制DNA合成和增殖；(2)细胞扩散增加的形态学改变；或(3)刺激合成48千道尔顿分泌蛋白。然而，耐药细胞确实保留了TGF - β受体的功能。TGF - β耐药细胞系可能在基因研究中有用，旨在确定该因子抑制上皮细胞生长所需的生化事件。

Mink lung epithelial cells resistant to growth inhibition by transforming growth factor β (TGF‐β) have been isolated by chemical mutagenesis and growth in the presence of platelet extracts enriched in TGF‐β. Several resistant clones were isolated, at least one of which stably retained its resistance to TGF‐β when grown in the absence of the factor. The cells of this clone were similar to the parent cells in morphology and growth properties. However, unlike the parent cells, the resistant cells did not show any of the following responses to TGF‐β: (1) inhibition of DNA synthesis and proliferation; (2) morphological changes involving increased cell spreading; or (3) stimulation of synthesis of a 48‐kilodalton secreted protein. The resistant cells do, however, retain a functional TGF‐β receptor. The TGF‐β resistant cell lines may be useful in genetic studies designed to identify the biochemical events required for inhibition of epithelial cell growth by this factor.