The acute effects of light on murine sleep during the dark phase: importance of melanopsin for maintenance of light-induced sleep.
The acute effects of light on murine sleep during the dark phase: importance of melanopsin for maintenance of light-induced sleep.
复制标题
在黑暗阶段，光对鼠睡眠的急性影响：黑色素蛋白在维持光引起的睡眠方面的重要性。
DOI：
10.1111/ejn.12189
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Heller HC
中科院分区：
文献类型：
--
作者：
Muindi F;Zeitzer JM;Colas D;Heller HC
光对夜间和白天活动的动物的睡眠和觉醒有直接影响，在黑暗阶段的光脉冲抑制运动活动并促进前者的睡眠。在本研究中，我们通过在黑暗阶段（Zeitgeber时间13-14）将小鼠暴露于宽范围的照度（0.2-200 μW/cm 2;相当于1-1000勒克斯）1小时来研究光对各种睡眠参数的直接影响。用三参数对数模型拟合数据表明，约0.1 μW/cm 2可以产生200 μW/cm 2时观察到的睡眠反应的一半。我们观察到在光脉冲结束后的1小时内总睡眠时间减少。光照使睡眠潜伏期从黑暗中的约30分钟（基线）减少到最高强度下的约10分钟，尽管这种效果在所使用的光照强度下是不变的。然后，我们评估了黑视素在光脉冲开始时从觉醒到睡眠的快速过渡过程中的作用，以及用6小时20 μW/cm 2光脉冲维持睡眠的作用。尽管黑视素敲除小鼠在脉冲的第一个小时内能够强烈诱导睡眠（约35分钟），但它并没有维持。与脉冲的第一个小时相比，缺乏黑视蛋白的小鼠在第三个小时的总睡眠减少了近65%，而在野生型小鼠中只观察到8%的减少。总的来说，我们的研究结果强调了光对小鼠睡眠的选择性影响，并表明基于黑视蛋白的光感受主要参与维持光诱导的睡眠。
Light exerts a direct effect on sleep and wakefulness in nocturnal and diurnal animals, with a light pulse during the dark phase suppressing locomotor activity and promoting sleep in the former. In the present study, we investigated this direct effect of light on various sleep parameters by exposing mice to a broad range of illuminances (0.2–200 μW/cm2; equivalent to 1–1000 lux) for 1 h during the dark phase (zeitgeber time 13–14). Fitting the data with a three-parameter log model indicated that ~0.1 μW/cm2 can generate half the sleep response observed at 200 μW/cm2. We observed decreases in total sleep time during the 1 h following the end of the light pulse. Light reduced the latency to sleep from ~30 min in darkness (baseline) to ~10 min at the highest intensity, although this effect was invariant across the light intensities used. We then assessed the role of melanopsin during the rapid transition from wakefulness to sleep at the onset of a light pulse and the maintenance of sleep with a 6-h 20 μW/cm2 light pulse. Even though the melanopsin knockout mice had robust induction of sleep (~35 min) during the first hour of the pulse, it was not maintained. Total sleep decreased by almost 65% by the third hour in comparison with the first hour of the pulse in mice lacking melanopsin, whereas only an 8% decrease was observed in wild-type mice. Collectively, our findings highlight the selective effects of light on murine sleep, and suggest that melanopsin-based photoreception is primarily involved in sustaining light-induced sleep.
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影响因子：
25
作者：
Altimus, Cara M.;Gueler, Ali D.;Alam, Nazia M.;Arman, A. Cyrus;Prusky, Glen T.;Sampath, Alapakkam P.;Hattar, Samer
通讯作者：
Hattar, Samer
影响因子：
2.9
作者：
MEIJER, JH;RUSAK, B;GANSHIRT, G
通讯作者：
GANSHIRT, G
影响因子：
6.1
作者：
Colas, Damien;Valletta, Janice S.;Mignot, Emmanuel
通讯作者：
Mignot, Emmanuel
影响因子：
3.4
作者：
Deboer, Tom;Ruijgrok, Guido;Meijer, Johanna H.
通讯作者：
Meijer, Johanna H.
影响因子：
25
作者：
Deboer, T;Vansteensel, MJ;Meijer, JH
通讯作者：
Meijer, JH