Survey of X-ray induced Cherenkov excited fluorophores with potential for human use.
Survey of X-ray induced Cherenkov excited fluorophores with potential for human use.
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DOI：
10.1093/jrr/rrab055
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发表时间：
2021-09-13
影响因子：
2
通讯作者：
Pogue BW
中科院分区：
文献类型：
--
作者：
Pétusseau AF;Bruza P;Pogue BW
X 射线诱导分子发光 (XML) 是一种可用于定制分子探针的临床深层组织功能成像的现象。在这项研究中，对常见或临床认可的荧光团的兆伏 X 射线诱导激发和发射特性进行了调查。我们发现直接闪烁效应和切伦科夫产生是引起这些分子激发的两种可能方式。为了区分每种激发机制的贡献，我们利用了切伦科夫辐射产量对 X 射线能量的依赖性。探针接受恒定剂量的 6 MV 和 18 MV X 射线辐射，并对每个 X 射线能量对的相对发射强度和光谱进行量化。从 XML 的比率、6 MV 和 18 MV 辐射的产额，我们发现切伦科夫辐射作为激发机制占主导地位，但铝酞菁除外，铝酞菁表现出明显的闪烁。检测到最高发射率的依次为荧光素、丙黄素和铝酞菁。发现 XML 产率受到发射量子产率、荧光激发和切伦科夫发射光谱的重叠、闪烁产率的影响。考虑到所有这些因素以及与组织光学窗口相关的 XML 发射光谱，铝酞菁为深层组织使用提供了最佳的 XML 产率，而荧光素和原黄素对于皮下或浅表使用最有用。
X-ray induced molecular luminescence (XML) is a phenomenon that can be utilized for clinical, deep-tissue functional imaging of tailored molecular probes. In this study, a survey of common or clinically approved fluorophores was carried out for their megavoltage X-ray induced excitation and emission characteristics. We find that direct scintillation effects and Cherenkov generation are two possible ways to cause these molecules’ excitation. To distinguish the contributions of each excitation mechanism, we exploited the dependency of Cherenkov radiation yield on X-ray energy. The probes were irradiated by constant dose of 6 MV and 18 MV X-ray radiation, and their relative emission intensities and spectra were quantified for each X-ray energy pair. From the ratios of XML, yield for 6 MV and 18 MV irradiation we found that the Cherenkov radiation dominated as an excitation mechanism, except for aluminum phthalocyanine, which exhibited substantial scintillation. The highest emission yields were detected from fluorescein, proflavin and aluminum phthalocyanine, in that order. XML yield was found to be affected by the emission quantum yield, overlap of the fluorescence excitation and Cherenkov emission spectra, scintillation yield. Considering all these factors and XML emission spectrum respective to tissue optical window, aluminum phthalocyanine offers the best XML yield for deep tissue use, while fluorescein and proflavine are most useful for subcutaneous or superficial use.
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影响因子：
--
作者：
Zhegalova, Natalia G.;He, Shawn;Zhou, Haiying;Kim, David M.;Berezin, Mikhail Y.
通讯作者：
Berezin, Mikhail Y.
体内纳米颗粒介导的放射性药物激发荧光分子成像
影响因子：
16.6
作者：
Hu, Zhenhua;Qu, Yawei;Wang, Kun;Zhang, Xiaojun;Zha, Jiali;Song, Tianming;Bao, Chengpeng;Liu, Haixiao;Wang, Zhongliang;Wang, Jing;Liu, Zhongyu;Liu, Haifeng;Tian, Jie
通讯作者：
Tian, Jie
影响因子：
3.8
作者：
KUBOTA, Y;STEINER, RF
通讯作者：
STEINER, RF
影响因子：
3.5
作者：
Lin, Huiyun;Zhang, Rongxiao;Pogue, Brian W.
通讯作者：
Pogue, Brian W.
影响因子：
3.6
作者：
Pratx, Guillem;Carpenter, Colin M.;Xing, Lei
通讯作者：
Xing, Lei