Aerobactin synthesis genes iucA and iucC contribute to the pathogenicity of avian pathogenic Escherichia coli O2 strain E058.
Aerobactin synthesis genes iucA and iucC contribute to the pathogenicity of avian pathogenic Escherichia coli O2 strain E058.
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DOI：
10.1371/journal.pone.0057794
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Ling J;Pan H;Gao Q;Xiong L;Zhou Y;Zhang D;Gao S;Liu X
已知产气菌素基因存在于强毒株中，而在无毒毒株中不存在，但是参与产气菌素合成的iucC和iucA对禽致病性大肠杆菌（APEC）的致病性的贡献尚未阐明。在这项研究中，双突变体（iucA/iutA或iucC/iutA）相比，单突变体（iucA，iucC或iutA）对APEC菌株E058的毒力的影响，在体外（产气菌素，摄入HD-11细胞，在鸡血清中的存活）和体内（与亲本菌株的竞争性生长，定殖和持久性）进行了检查。在竞争性共感染试验中，与E058亲本菌株相比，E058ΔiucA突变体在肝、肾、脾（均P<0.01）、心脏和肺（均P<0.001）中显著减少。E058ΔiutA突变体在肝、肺、肾（均P<0.01）、心和脾（均P<0.001）中的表达也显著降低。E058ΔiucC突变体在心、肾组织中的表达明显减弱（均P<0.05），在肝、脾、肺组织中的表达明显减少（P<0.01），同时E058ΔiucAΔiutA和E058ΔiucCΔiutA双突变体的表达也明显减少（P<0.001）。与E058相比，E058ΔiucA在肺、肝、脾和肾中的克隆数显著减少（P<0.01），在心脏中的克隆数显著减少（P<0.001）。E058ΔiutA在心、肺、肝、脾、肾组织中均显著降低（P<0.01）。E058ΔiucC、E058ΔiucAΔiutA和E058ΔiucCΔiutA在各脏器中均显著降低（P<0.001）。这些结果表明，iutA，iucA和iucC在APEC E058的致病性中起重要作用。
Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been clarified. In this study, effects of double mutants (iucA/iutA or iucC/iutA) compared to those of single mutants (iucA, iucC or iutA) of aerobactin genes on the virulence of APEC strain E058 were examined both in vitro (aerobactin production, ingestion into HD-11 cells, survival in chicken serum) and in vivo (competitive growth against parental strain, colonization and persistence). In competitive co-infection assays, compared to the E058 parental strain, the E058ΔiucA mutant was significantly reduced in the liver, kidney, spleen (all P<0.01), heart and lung (both P<0.001). The E058ΔiutA mutant also was significantly reduced in the liver, lung, kidney (all P<0.01), heart and spleen (both P<0.001). The E058ΔiucC mutant was significantly attenuated in the heart and kidney (both P<0.05) and showed a remarkable reduction in the liver, spleen and lung (P<0.01); meanwhile, both E058ΔiucAΔiutA and E058ΔiucCΔiutA double mutants were sharply reduced as well (P<0.001). In colonization and persistence assays, compared with E058, recovered colonies of E058ΔiucA were significantly reduced from the lung, liver, spleen and kidney (P<0.01) and significantly reduced in the heart (P<0.001). E058ΔiutA was significantly reduced from the heart, lung, liver, spleen and kidney (P<0.01). E058ΔiucC, E058ΔiucAΔiutA and E058ΔiucCΔiutA were significantly decreased in all organs tested (P<0.001). These results suggest that iutA, iucA and iucC play important roles in the pathogenicity of APEC E058.
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影响因子：
3.2
作者：
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通讯作者：
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