Mechanical and cell viability properties of crosslinked low- and high-molecular weight poly(ethylene glycol) diacrylate blends.
Mechanical and cell viability properties of crosslinked low- and high-molecular weight poly(ethylene glycol) diacrylate blends.
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DOI：
10.1002/jbm.a.32563
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发表时间：
2010-05
影响因子：
4.9
通讯作者：
Pintauro, Peter N.
中科院分区：
文献类型：
--
作者：
Mazzoccoli, Jason P.;Feke, Donald L.;Baskaran, Harihara;Pintauro, Peter N.
对于机械坚固、表现出良好的生物相容性并且可以由容易获得的材料制成的组织工程支架存在强烈的需求。为此，将分子量为400和3400的市售聚（乙二醇）二丙烯酸酯（PEGDA）的共混物在系统地从20至40重量%变化的总聚合物浓度下进行UV交联。测定每种PEGDA混合物的抗压强度和细胞活力。当PEGDA 3400/400的质量比为40/60时，共混物的压缩模量最大，压缩强度达到1.7 MPa。活/死荧光测定的细胞活力结果显示，对于所有共混物，在20重量%的总PEGDA浓度下，平均活力为约80%。增加总聚合物浓度增加聚合物的压缩模量，但不利地影响所有PEGDA共混物组合物的细胞活力。共混物的组成影响的机械性能的光盘，其中更高程度的交联是通过增加浓度的较短链的PEGDA 400，而弹性获得通过将较长链的PEGDA 3400的共混物。这些结果可用于组织工程应用，其中机械坚固的支架是有利的。
There is a strong need for tissue engineering scaffolds that are mechanically robust, exhibit good biocompatibility, and can be made from readily available materials. To this end, blends of commercially available poly(ethylene glycol) diacrylate (PEGDA) with molecular weights of 400 and 3400 were UV-crosslinked at total polymer concentrations that varied systematically from 20 to 40 wt %. The compressive strength and cell viability were determined for each PEGDA mixture. The compressive modulus of the blends was maximized when the wt % ratio PEGDA3400/400 was about 40/60, with the compressive strength reaching 1.7 MPa. Cell viability results with a LIVE/DEAD fluorescence assay show an average viability of ~ 80% at a total PEGDA concentration of 20 wt %, for all blends. Increasing the total polymer concentration increased the compressive modulus of a polymer, but adversely affected cell viability for all the PEGDA blend compositions. The blend composition affected the mechanical behavior of the discs, where a higher degree of crosslinking was achieved by increasing the concentration of shorter chained PEGDA400, whereas elasticity was gained by incorporating longer chained PEGDA3400 into the blends. These results can be exploited for use in tissue engineering applications, where a mechanically robust scaffold is advantageous.
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