A ribosomal protein AgRPS3aE from halophilic Aspergillus glaucus confers salt tolerance in heterologous organisms.
A ribosomal protein AgRPS3aE from halophilic Aspergillus glaucus confers salt tolerance in heterologous organisms.
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DOI：
10.3390/ijms16023058
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发表时间：
2015-01-29
影响因子：
5.6
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Liang X;Liu Y;Xie L;Liu X;Wei Y;Zhou X;Zhang S
尽管盐碱地被认为是潜在的农业可耕资源，但土壤高盐是显著降低作物产量的非生物胁迫之一。目前，提高耐盐性的基因工程正在作为一种有效和可行的作物改良策略进行试验。我们之前描述了核糖体蛋白RPL44的一个大亚基，它参与了极端嗜盐真菌青绿Aspergillus glaucus的渗透胁迫。在这里，我们筛选了另一种在酵母中也产生高盐耐受性的核糖体蛋白（AgRPS3aE）。生物信息学分析表明，agps3ae编码真核生物中属于RPS3Ae家族的核糖体蛋白的一个29.2 kDa小亚基。为了进一步证实其对盐胁迫的保护作用，我们在3个异源系统、丝状真菌Magnaporthe oryzae和2种模式植物拟南芥和烟草中表达了AgRPS3aE。与对照相比，在所有测试的转化体中过表达AgRPS3aE显著缓解了胁迫症状，这表明AgRPS3aE不仅在真菌中起作用，而且在植物中也起作用。考虑到核糖体蛋白是生物从原核生物到真核生物的清洁成分，我们认为AgRPS3aE是提高作物耐高盐性的最佳基因之一。
High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops.
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影响因子：
7.4
作者：
Falcone Ferreyra, Maria Lorena;Pezza, Alejandro;Casati, Paula
通讯作者：
Casati, Paula
非生物胁迫抗性，核糖体蛋白 RPL44 在嗜盐真菌灰绿曲霉中的新兼职功能
影响因子：
4.4
作者：
Liu, Xiao-Dan;Xie, Lixia;Zhang, Shihong
通讯作者：
Zhang, Shihong
影响因子：
4
作者：
Naora, H;Naora, H
通讯作者：
Naora, H
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Mok, Young Geun;Lee, Byoung Doo;Hwang, Seongbin
通讯作者：
Hwang, Seongbin