SARS-Cov-2 ORF3a: Mutability and function.
SARS-Cov-2 ORF3a: Mutability and function.
复制标题
DOI：
10.1016/j.ijbiomac.2020.12.142
复制
发表时间：
2021-02-15
影响因子：
8.2
通讯作者：
Pascarella S
中科院分区：
文献类型：
--
作者：
Bianchi M;Borsetti A;Ciccozzi M;Pascarella S
本研究分析了SARS-CoV-2 ORF3a蛋白在大流行过程中的变化。ORF3a是一种保守的冠状病毒蛋白，参与病毒的复制和释放。截至2020年8月底，GISAID数据库中可获得的一组70,752个高质量的SARS-CoV-2基因组已被扫描。根据收集日期间隔和整个数据集对病毒基因组中的所有ORF3a突变进行分组。审议的时间间隔为：开始收集--2020年2月、3月、4月、5月、6月、7月和8月。在每个收集间隔内检查了最常见的前五个变异。总体而言，已分离出17个变种。17个突变位点中有10个发生在ORF3a的跨膜(TM)结构域内，并与中央孔道或侧隧道接触。其他变异位点位于ORF3a结构的不同位置。在整个样本中，最常见的五个突变是V13L、Q57H、Q57H+、A99V、G196V和G252V。同样的分析确定了28个在所有基因组分离株中相同保守的位点。这些位点可能参与单体、二聚体、四聚体的稳定以及与其他细胞成分的相互作用。本文报道的结果有助于了解病毒生物学和设计新的治疗策略。
In this study, analysis of changes of SARS-CoV-2 ORF3a protein during pandemic is reported. ORF3a, a conserved coronavirus protein, is involved in virus replication and release. A set of 70,752 high-quality SARS-CoV-2 genomes available in GISAID databank at the end of August 2020 have been scanned. All ORF3a mutations in the virus genomes were grouped according to the collection date interval and over the entire data set. The considered intervals were: start of collection-February, March, April, May, June, July and August 2020. The top five most frequent variants were examined within each collection interval. Overall, seventeen variants have been isolated. Ten of the seventeen mutant sites occur within the transmembrane (TM) domain of ORF3a and are in contact with the central pore or side tunnels. The other variant sites are in different places of the ORF3a structure. Within the entire sample, the five most frequent mutations are V13L, Q57H, Q57H + A99V, G196V and G252V. The same analysis identified 28 sites identically conserved in all the genome isolates. These sites are possibly involved in stabilization of monomer, dimer, tetramerization and interaction with other cellular components. The results here reported can be helpful to understand virus biology and to design new therapeutic strategies.
登录
查看更多内容
影响因子：
3.4
作者：
Benvenuto, Domenico;Giovanetti, Marta;Ciccozzi, Massimo
通讯作者：
Ciccozzi, Massimo
影响因子：
--
作者：
Bianchi, Martina;Benvenuto, Domenico;Pascarella, Stefano
通讯作者：
Pascarella, Stefano
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
5.2
作者：
Liu S;Shen J;Fang S;Li K;Liu J;Yang L;Hu CD;Wan J
通讯作者：
Wan J
影响因子：
6.4
作者：
Castaño-Rodriguez C;Honrubia JM;Gutiérrez-Álvarez J;DeDiego ML;Nieto-Torres JL;Jimenez-Guardeño JM;Regla-Nava JA;Fernandez-Delgado R;Verdia-Báguena C;Queralt-Martín M;Kochan G;Perlman S;Aguilella VM;Sola I;Enjuanes L
通讯作者：
Enjuanes L