Polarity in migrating neurons is related to a mechanism analogous to cytokinesis.
Polarity in migrating neurons is related to a mechanism analogous to cytokinesis.
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迁移神经元的极性与类似于细胞分裂的机制有关
DOI：
10.1016/j.cub.2013.05.027
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发表时间：
2013-07-08
期刊：
影响因子：
9.2
通讯作者：
Baas, Peter W.
中科院分区：
文献类型：
--
作者：
Falnikar, Aditi;Tole, Shubha;Liu, Mei;Liu, Judy S.;Baas, Peter W.
迁移神经元是双极的，具有前导过程和尾随过程。引导过程的近端区域显示出有助于胞体和中心体前进的 F-肌动蛋白浓度。在这里，我们发现驱动蛋白 6（一种基于微管的运动蛋白，因其在胞质分裂中的作用而闻名）也集中在该区域。驱动蛋白 6 的耗尽会导致多极神经元静止或不断改变其运动方向。在这样的神经元中，F-肌动蛋白不再集中在单个过程中。在胞质分裂期间，kinesin-6 与称为 MgcRacGAP 的 Rho 家族 GTPase 激活蛋白形成复合物，向肌动蛋白细胞骨架发出信号，使皮质运动集中在卵裂沟中。在神经元迁移过程中，MgcRacGap 也集中在前导过程的近端区域，抑制其活性会导致类似于驱动蛋白 6 耗尽的表型。我们得出的结论是，神经元迁移利用类似于胞质分裂的细胞骨架途径，驱动蛋白 6 信号通过 MgcRacGap 发送到肌动蛋白细胞骨架，以限制突起数量并将突出活动限制在单个主导突起上，从而导致双极神经元能够以定向方式移动。
Migrating neurons are bipolar, with a leading process and a trailing process. The proximal region of the leading process displays a concentration of F-actin that contributes to the advance of the soma and the centrosome. Here we show that kinesin-6, a microtubule-based motor protein best known for its role in cytokinesis, also concentrates in this region. Depletion of kinesin-6 results in multipolar neurons that are either stationary or continuously change their direction of movement. In such neurons, F-actin no longer concentrates in a single process. During cytokinesis, kinesin-6 forms a complex with a Rho family GTPase activating protein called MgcRacGAP to signal to the actin cytoskeleton so that cortical movements are concentrated in the cleavage furrow. During neuronal migration, MgcRacGap also concentrates in the proximal region of the leading process, and inhibition of its activity results in a phenotype similar to kinesin-6 depletion. We conclude that neuronal migration utilizes a cytoskeletal pathway analogous to cytokinesis, with kinesin-6 signaling through MgcRacGap to the actin cytoskeleton to constrain process number and restrict protrusive activity to a single leading process, thus resulting in a bipolar neuron able to move in a directed fashion.
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鉴定微管相关的运动蛋白对于树突分化必不可少。
影响因子：
7.8
作者：
Sharp, DJ;Yu, WQ;Ferhat, L;Kuriyama, R;Rueger, DC;Baas, PW
通讯作者：
Baas, PW
DOI：
10.1083/jcb.200109090
发表时间：
2002-03-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kuriyama R;Gustus C;Terada Y;Uetake Y;Matuliene J
通讯作者：
Matuliene J
影响因子：
64.8
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR
影响因子：
3.3
作者：
Pavicic-Kaltenbrunner, Visnja;Mishima, Masanori;Glotzer, Michael
通讯作者：
Glotzer, Michael
影响因子：
11.8
作者：
Somers, WG;Saint, R
通讯作者：
Saint, R