Repair of segmental bone defect using Totally Vitalized tissue engineered bone graft by a combined perfusion seeding and culture system.
Repair of segmental bone defect using Totally Vitalized tissue engineered bone graft by a combined perfusion seeding and culture system.
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通过结合灌注接种和培养系统，使用完全活化的组织工程骨移植修复节段性骨缺损。
DOI：
10.1371/journal.pone.0094276
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lei W
中科院分区：
文献类型：
--
作者：
Wang L;Ma XY;Zhang Y;Feng YF;Li X;Hu YY;Wang Z;Ma ZS;Lei W
将成骨细胞与三维支架材料联合收割机复合是构建组织工程化骨移植物的基本策略。基于这一策略，我们提出了一种细胞数量丰富、分布均匀、增殖分化能力增强的“全活性TEBG”（TV-TEBG），并进一步研究了其修复节段性骨缺损的生物学性能。本研究采用快速成型技术（RP）制备β-磷酸三钙（β-TCP）支架，结合定制的流动灌注接种/培养系统，构建TV-TEBG。比较了灌注接种和灌注培养（PSPC）法、静态接种和灌注培养（SSPC）法、静态接种和静态培养（SSSC）法3种方法构建的TEBG的体外性能和兔骨缺损修复效果。我们的研究表明，PSPC法构建的TEBG具有更好的生物学特性，具有更高的每日D-葡萄糖消耗量，更高的细胞增殖和分化，以及更好的细胞分布，表明TV-TEBG的成功构建。植入兔桡骨缺损12周后，PSPC组X线评分接近自体骨，生物力学测试显示其力学性能明显优于其他两组，组织学分析显示其新骨形成明显高于其他两组，最终获得了最接近自体骨移植的节段性骨缺损良好的愈合效果。本研究证明了灌注接种、灌注培养和RP技术相结合构建TV-TEBG的可行性，并显示出良好的生物学特性。TV-TEBG的应用有望成为骨科颌面部节段性骨缺损修复的首选材料。
The basic strategy to construct tissue engineered bone graft (TEBG) is to combine osteoblastic cells with three dimensional (3D) scaffold. Based on this strategy, we proposed the “Totally Vitalized TEBG” (TV-TEBG) which was characterized by abundant and homogenously distributed cells with enhanced cell proliferation and differentiation and further investigated its biological performance in repairing segmental bone defect. In this study, we constructed the TV-TEBG with the combination of customized flow perfusion seeding/culture system and β-tricalcium phosphate (β-TCP) scaffold fabricated by Rapid Prototyping (RP) technique. We systemically compared three kinds of TEBG constructed by perfusion seeding and perfusion culture (PSPC) method, static seeding and perfusion culture (SSPC) method, and static seeding and static culture (SSSC) method for their in vitro performance and bone defect healing efficacy with a rabbit model. Our study has demonstrated that TEBG constructed by PSPC method exhibited better biological properties with higher daily D-glucose consumption, increased cell proliferation and differentiation, and better cell distribution, indicating the successful construction of TV-TEBG. After implanted into rabbit radius defects for 12 weeks, PSPC group exerted higher X-ray score close to autograft, much greater mechanical property evidenced by the biomechanical testing and significantly higher new bone formation as shown by histological analysis compared with the other two groups, and eventually obtained favorable healing efficacy of the segmental bone defect that was the closest to autograft transplantation. This study demonstrated the feasibility of TV-TEBG construction with combination of perfusion seeding, perfusion culture and RP technique which exerted excellent biological properties. The application of TV-TEBG may become a preferred candidate for segmental bone defect repair in orthopedic and maxillofacial fields.
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影响因子：
--
作者：
Ma, PX;Choi, JW
通讯作者：
Choi, JW
影响因子：
14
作者：
Ge, ZG;Baguenard, S;Khor, E
通讯作者：
Khor, E
影响因子：
14
作者：
Goldstein, AS;Juarez, TM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
4.8
作者：
McGarry, JG;Klein-Nulend, J;Prendergast, PJ
通讯作者：
Prendergast, PJ
DOI：
10.1023/a:1011203226053
发表时间：
2001-01-01
影响因子：
3.7
作者：
Chu, TMG;Halloran, JW;Feinberg, SE
通讯作者：
Feinberg, SE