Identification of a predicted trimeric autotransporter adhesin required for biofilm formation of Burkholderia pseudomallei.
Identification of a predicted trimeric autotransporter adhesin required for biofilm formation of Burkholderia pseudomallei.
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DOI：
10.1371/journal.pone.0079461
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Galyov EE
中科院分区：
文献类型：
--
作者：
Lazar Adler NR;Dean RE;Saint RJ;Stevens MP;Prior JL;Atkins TP;Galyov EE
自转运蛋白是一个庞大而多样的细菌分泌和外膜蛋白家族，存在于许多革兰氏阴性细菌病原体中，并在许多环境和毒力相关的相互作用中发挥作用。作为一个更大的系统性研究的一部分，类鼻疽伯克霍尔德氏菌，严重的热带疾病类鼻疽的病原体的自体转运蛋白，我们已经构建了一个插入突变体的bpss1439基因编码一个未经研究的预测三聚体自体转运蛋白粘附素。与其亲本10276野生型菌株相比，bpss1439突变体在48小时时表现出生物膜形成的显著减少。这种表型的补充野生型水平的bpss1439基因的全长拷贝的反式引入。检查的野生型和bpss1439突变株下的生物膜诱导条件下通过显微镜检查48小时后证实，bpss1439突变体产生的生物膜相比，野生型。此外，观察到这种表型是由于低水平的细菌粘附到非生物表面以及减少的小菌落形成。在鼠类鼻疽模型中，与野生型菌株相比，bpss1439突变菌株表现出中等毒力衰减。这种衰减被反式互补消除，表明bpss1439在B的发病机制中起着微妙的作用。假鼻疽总而言之，这些研究表明BPSS 1439是一种新型预测的自身转运蛋白，参与B的生物膜形成。因此，该因子被命名为BbfA，伯克霍尔德氏菌生物膜因子A。
The autotransporters are a large and diverse family of bacterial secreted and outer membrane proteins, which are present in many Gram-negative bacterial pathogens and play a role in numerous environmental and virulence-associated interactions. As part of a larger systematic study on the autotransporters of Burkholderia pseudomallei, the causative agent of the severe tropical disease melioidosis, we have constructed an insertion mutant in the bpss1439 gene encoding an unstudied predicted trimeric autotransporter adhesin. The bpss1439 mutant demonstrated a significant reduction in biofilm formation at 48 hours in comparison to its parent 10276 wild-type strain. This phenotype was complemented to wild-type levels by the introduction of a full-length copy of the bpss1439 gene in trans. Examination of the wild-type and bpss1439 mutant strains under biofilm-inducing conditions by microscopy after 48 hours confirmed that the bpss1439 mutant produced less biofilm compared to wild-type. Additionally, it was observed that this phenotype was due to low levels of bacterial adhesion to the abiotic surface as well as reduced microcolony formation. In a murine melioidosis model, the bpss1439 mutant strain demonstrated a moderate attenuation for virulence compared to the wild-type strain. This attenuation was abrogated by in trans complementation, suggesting that bpss1439 plays a subtle role in the pathogenesis of B. pseudomallei. Taken together, these studies indicate that BPSS1439 is a novel predicted autotransporter involved in biofilm formation of B. pseudomallei; hence, this factor was named BbfA, Burkholderia biofilm factor A.
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影响因子：
5.2
作者：
Lazar Adler NR;Stevens JM;Stevens MP;Galyov EE
通讯作者：
Galyov EE
影响因子：
3.1
作者：
Chanchamroen, Sujin;Kewcharoenwong, Chidchamai;Lertmemongkolchai, Ganjana
通讯作者：
Lertmemongkolchai, Ganjana
DOI：
10.1038/nbt1183-784
发表时间：
1983-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
SIMON, R;PRIEFER, U;PUHLER, A
通讯作者：
PUHLER, A
影响因子：
3.7
作者：
Mil-Homens D;Fialho AM
通讯作者：
Fialho AM
影响因子：
3
作者：
Atkins, T;Prior, R;Titball, RW
通讯作者：
Titball, RW