Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice.
Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice.
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DOI：
10.1007/s00441-012-1490-9
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发表时间：
2012-12
影响因子：
3.6
通讯作者：
Bingle, Colin D.
中科院分区：
文献类型：
--
作者：
Musa, Maslinda;Wilson, Kirsty;Sun, Le;Mulay, Apoorva;Bingle, Lynne;Marriott, Helen M.;LeClair, Elizabeth E.;Bingle, Colin D.
尽管最初是在小鼠中发现的，但对于该物种中含有（PLUNC）家族的BPI折叠（BPIF）成员的产生位点知之甚少，这些家族被认为是先天防御蛋白。这些蛋白在很大程度上被认为是在呼吸道特异性表达的，我们最近发现它们在近端气道上皮中表现出差异表达。在这项研究中，我们使用物种特异性抗体系统地定位了该蛋白家族的两个成员；成年小鼠的BPIFA1 （PLUNC/SPLUNC1）和BPIFB1 （LPLUNC1）。一般来说，这些蛋白表现出明显的和部分重叠的定位。BPIFA1在鼻通道的呼吸上皮和鲍曼腺中高表达，而BPIFB1则存在于鼻通道和咽部的一小部分杯状细胞中。BPIFB1也存在于舌近端浆液腺中，与唾液腺特异性家族成员BPIFA2E（腮腺分泌蛋白）共定位，也存在于软腭腺体中。这两种蛋白在这些区域之外都表现出有限的表达。这些结果与在人体内观察到的蛋白质定位一致。了解BPIF蛋白在这些区域的复杂表达模式，将允许使用可处理的小鼠疾病模型来解剖其功能。本文的在线版本（doi:10.1007/s00441-012-1490-9）包含补充材料，授权用户可使用。
Despite being initially identified in mice, little is known about the sites of production of members of the BPI fold (BPIF) containing (PLUNC) family of putative innate defence proteins in this species. These proteins have largely been considered to be specificaly expressed in the respiratory tract, and we have recently shown that they exhibit differential expression in the epithelium of the proximal airways. In this study, we have used species-specific antibodies to systematically localize two members of this protein family; BPIFA1 (PLUNC/SPLUNC1) and BPIFB1 (LPLUNC1) in adult mice. In general, these proteins exhibit distinct and only partially overlapping localization. BPIFA1 is highly expressed in the respiratory epithelium and Bowman’s glands of the nasal passages, whereas BPIFB1 is present in small subset of goblet cells in the nasal passage and pharynx. BPIFB1 is also present in the serous glands in the proximal tongue where is co-localised with the salivary gland specific family member, BPIFA2E (parotid secretory protein) and also in glands of the soft palate. Both proteins exhibit limited expression outside of these regions. These results are consistent with the localization of the proteins seen in man. Knowledge of the complex expression patterns of BPIF proteins in these regions will allow the use of tractable mouse models of disease to dissect their function. The online version of this article (doi:10.1007/s00441-012-1490-9) contains supplementary material, which is available to authorized users.
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