Innovations present in the primate interneuron repertoire.
Innovations present in the primate interneuron repertoire.
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DOI：
10.1038/s41586-020-2781-z
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发表时间：
2020-10
期刊：
影响因子：
64.8
通讯作者：
McCarroll SA
中科院分区：
文献类型：
--
作者：
Krienen FM;Goldman M;Zhang Q;C H Del Rosario R;Florio M;Machold R;Saunders A;Levandowski K;Zaniewski H;Schuman B;Wu C;Lutservitz A;Mullally CD;Reed N;Bien E;Bortolin L;Fernandez-Otero M;Lin JD;Wysoker A;Nemesh J;Kulp D;Burns M;Tkachev V;Smith R;Walsh CA;Dimidschstein J;Rudy B;S Kean L;Berretta S;Fishell G;Feng G;McCarroll SA
灵长类和啮齿动物是大约9000万年前共同祖先的后代，它们在行为和认知能力上表现出深刻的差异；这些差异的细胞基础尚不清楚。在这里，我们使用单核RNA测序来分析来自三种灵长类动物(人、猕猴和绒猴)、一种啮齿动物(小鼠)和一只黄鼠狼(雪貂)的188,776个个体中间神经元的RNA表达。同源的中间神经元类型--很容易通过它们的RNA表达模式来识别--在雪貂、小鼠和灵长类动物中的丰度和RNA表达有所不同，但在灵长类动物中差异较小。在任何一个物种中，只有一小部分被识别为特定中间神经元亚型的‘标记’基因在另一个物种中具有这种特性。在灵长类新皮质中，数十个基因在同一类型的中间神经元之间表现出空间表达梯度，这表明皮质环境的区域差异塑造了成年新皮质中间神经元的RNA表达模式。我们发现，一种以前与小鼠海马体有关的中间神经元类型-具有神经胶质样特征的常春藤细胞-已经在人类、猕猴和绒猴的新皮质中变得丰富，但不包括老鼠或雪貂。我们还发现了一个显着的皮质下创新：灵长类动物中丰富的纹状体中间神经元类型，而在小鼠或雪貂中没有分子上的同源对应类型。这些中间神经元表达编码转录因子、受体和神经肽的独特基因组合，约占绒猴和人类纹状体中间神经元的30%。
Primates and rodents, which descended from a common ancestor around 90 million years ago, exhibit profound differences in behaviour and cognitive capacity; the cellular basis for these differences is unknown. Here we use single-nucleus RNA sequencing to profile RNA expression in 188,776 individual interneurons across homologous brain regions from three primates (human, macaque and marmoset), a rodent (mouse) and a weasel (ferret). Homologous interneuron types–which were readily identified by their RNA-expression patterns–varied in abundance and RNA expression among ferrets, mice and primates, but varied less among primates. Only a modest fraction of the genes identified as ‘markers’ of specific interneuron subtypes in any one species had this property in another species. In the primate neocortex, dozens of genes showed spatial expression gradients among interneurons of the same type, which suggests that regional variation in cortical contexts shapes the RNA expression patterns of adult neocortical interneurons. We found that an interneuron type that was previously associated with the mouse hippocampus–the ‘ivy cell’, which has neurogliaform characteristics–has become abundant across the neocortex of humans, macaques and marmosets but not mice or ferrets. We also found a notable subcortical innovation: an abundant striatal interneuron type in primates that had no molecularly homologous counterpart in mice or ferrets. These interneurons expressed a unique combination of genes that encode transcription factors, receptors and neuropeptides and constituted around 30% of striatal interneurons in marmosets and humans.
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影响因子：
16.2
作者：
He M;Tucciarone J;Lee S;Nigro MJ;Kim Y;Levine JM;Kelly SM;Krugikov I;Wu P;Chen Y;Gong L;Hou Y;Osten P;Rudy B;Huang ZJ
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影响因子：
11.1
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DOI：
10.1038/s41583-019-0195-4
发表时间：
2019-09
期刊：
Nature reviews. Neuroscience
影响因子：
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作者：
Huang ZJ;Paul A
通讯作者：
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