Identification and characterization of pannexin expression in the mammalian cochlea.
Identification and characterization of pannexin expression in the mammalian cochlea.
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DOI：
10.1002/cne.21898
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发表时间：
2009-01-20
影响因子：
2.5
通讯作者：
Zhao, Hong-Bo
中科院分区：
文献类型：
--
作者：
Wang, Xiao-Hui;Streeter, Michele;Liu, Ying-Peng;Zhao, Hong-Bo
脊椎动物中的差距连接由连接蛋白基因家族编码。最近，在脊椎动物中发现了一个新的基因家族，称为泛连接蛋白（Panx），它也编码间隙连接蛋白。迄今为止，已经从小鼠和人类基因组中克隆了三种泛连接蛋白同种型（Panx 1、2和3）。在这项研究中，pannexins在小鼠和大鼠耳蜗的表达进行了研究。PCR和Western blot分析表明，所有三种泛连接蛋白亚型在耳蜗中表达。免疫荧光染色显示Panx1广泛表达。在Corti器中，Panx 1标记见于支持细胞，包括柱细胞、Hensen细胞、Claudius细胞和Boettcher细胞。表面空斑样点状标记和弥漫性胞质标记均可见。然而，在Deiters细胞中的标记是弱的和罕见的。在毛细胞中未发现标记。在螺旋利姆布斯的齿间细胞、内外沟细胞以及螺旋突起和耳蜗外侧壁中央区域的II型纤维细胞中也观察到Panx 1的强烈标记。然而，未观察到重叠标记。此外，Panx1标记可在Reissner膜和血管内皮细胞中检测到。Panx 2标记仅限于血管纹中的基底细胞，并且在螺旋神经节神经元中也可检测到。然而，没有观察到Panx1和Panx2的重叠标记。最后，Panx3标记仅在耳蜗骨中观察到。因此，Panx 1，2和3在哺乳动物耳蜗中大量表达，并表现出不同的细胞分布。与连接蛋白一样，它们可能在听力中发挥重要作用。
The gap junction in vertebrates is encoded by the connexin gene family. Recently, a new gene family termed pannexin (Panx) has been identified in vertebrates and found to encode gap junctional proteins as well. To date, three pannexin isoforms (Panx1, 2 and 3) have been cloned from mouse and human genomes. In this study, expression of pannexins in the mouse and rat cochlea was investigated. PCR and Western blot analysis showed that all three pannexin isoforms were expressed in the cochlea. Immunofluorescent staining showed that Panx1 expression was extensive. In the organ of Corti, Panx1 labeling was found in supporting cells, including pillar cells, Hensen cells, Claudius cells and Boettcher cells. Both surface plaque-like punctate labeling and diffuse-cytoplasmic labeling were visible. However, the labeling was weak and rare in Deiters cells. No labeling was found in the hair cells. Intense labeling for Panx1 was also observed in the interdental cells in the spiral limbus, the inner and outer sulcus cells, and the type II fibrocytes in the spiral prominence and central region in the cochlear lateral wall. However, no overlapping labeling was observed. In addition, Panx1 labeling was detectable in the Reissner's membrane and strial blood vessel cells. Panx2 labeling was restricted to the basal cells in the stria vascularis and was also detectable in the spiral ganglion neurons. However, no overlapping labeling for Panx1 and Panx2 was observed. Finally, Panx3 labeling was exclusively observed in the cochlear bone. Thus, Panx1, 2 and 3 are abundantly expressed in the mammalian cochlea and demonstrate distinct cellular distributions. Like connexins, they may play an important role in hearing.
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DOI：
10.1007/bf00186783
发表时间：
1995-02-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
KIKUCHI, T;KIMURA, RS;ADAMS, JC
通讯作者：
ADAMS, JC
DOI：
10.1073/pnas.2233464100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bruzzone, R;Hormuzdi, SG;Monyer, H
通讯作者：
Monyer, H
影响因子：
11.2
作者：
Lai, Charles P. K.;Bechberger, John F.;Naus, Christian C.
通讯作者：
Naus, Christian C.
DOI：
10.1073/pnas.0611280104
发表时间：
2007-04-10
影响因子：
11.1
作者：
Huang, Yi-Jen;Maruyama, Yutaka;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
影响因子：
3
作者：
Hurley, PA;Clarke, M;Shepherd, RK
通讯作者：
Shepherd, RK