Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia.
Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia.
复制标题
确定与成人罗非鱼大量死亡率相关的新型细节病毒病原体。
DOI：
10.1371/journal.ppat.1008765
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发表时间：
2020-09
期刊：
影响因子：
6.7
通讯作者：
Zeng L
中科院分区：
文献类型：
--
作者：
Liu W;Zhang Y;Ma J;Jiang N;Fan Y;Zhou Y;Cain K;Yi M;Jia K;Wen H;Liu W;Guan W;Zeng L
罗非鱼是世界上最重要的经济物种之一，也是水产养殖中增长最快的物种之一。2015年，湖北发生了一起与成鱼严重死亡相关的疫情，中国。通过病毒分离、电子显微镜、实验激发、原位杂交(ISH)、间接免疫荧光(IFA)和病毒基因序列测定，确定病原为罗非鱼细小病毒(Tilapia parvoVirus，TiPV)。电子显微镜观察发现，在病鱼的肾、脾、肝、心、脑、鳃、肠等器官中存在大量的细小病毒颗粒，这些病毒颗粒呈球形，无包膜，直径约30 nm。在罗非鱼脑细胞(TIB)中分离和繁殖TiPV，感染3d后出现典型的细胞病变(CPE)。这种病毒被用来实验感染成年罗非鱼，并复制了与自然观察到的临床症状相似的疾病症状。此外，在感染TiPV的鱼的肾和脾组织中，ISH和IFA结果显示出阳性信号。为了鉴定TiPV特异性序列，获得了TiPV的近完整基因组，并确定其大小为4269bp。NS1序列的系统发育分析表明，TiPV是一种新的细小病毒，属于细小病毒科ChapparvoVirus的一个独立分支。研究结果证实，TiPV是一种新的细小病毒病原体，可导致成年罗非鱼大量死亡。这为进一步研究确定这种新出现的病毒性疾病的流行病学、病理学、诊断、预防和治疗提供了基础。从成体罗非鱼中分离到一种新的细小病毒，可导致相当大的发病率和死亡率。利用SISPA-PCR和RACE技术，我们鉴定和鉴定了该细小病毒的4269个核苷酸。初步命名为罗非鱼细小病毒(TiPV)，据我们所知，这是细小病毒科中第一个被证明感染硬骨鱼宿主的假定成员。我们发现，BLASTX与其他病毒的核苷酸序列相似性搜索没有明显的匹配，而氨基酸序列比较表明，与其他细小病毒的氨基酸(AA)同源性约为34.6%~50.0%。感染不同门或门的宿主的细小病毒基因组之间的相似性表明需要更新先前提出的关于细小病毒起源的假设。我们的发现可能代表了解释病毒进化的新途径，并提示有必要进一步研究细小病毒的发病机制。
Tilapia is one of the most important economic and fastest-growing species in aquaculture worldwide. In 2015, an epidemic associated with severe mortality occurred in adult tilapia in Hubei, China. The causative pathogen was identified as Tilapia parvovirus (TiPV) by virus isolation, electron microscopy, experimental challenge, In situ hybridization (ISH), indirect immunofluorescence (IFA), and viral gene sequencing. Electron microscopy revealed large numbers of parvovirus particles in the organs of diseased fish, including kidney, spleen, liver, heart, brain, gill, intestine, etc. The virions were spherical in shape, non-enveloped and approximately 30nm in diameter. The TiPV was isolated and propagated in tilapia brain cells (TiB) and induced a typical cytopathic effect (CPE) after 3 days post-infection (dpi). This virus was used to experimentally infect adult tilapia and clinical disease symptoms similar to those observed naturally were replicated. Additionally, the results of ISH and IFA showed positive signals in kidney and spleen tissues from TiPV-infected fish. To identify TiPV-specific sequences, the near complete genome of TiPV was obtained and determined to be 4269 bp in size. Phylogenetic analysis of the NS1 sequence revealed that TiPV is a novel parvovirus, forms a separate branch in proposed genus Chapparvovirus of Parvoviridae. Results presented here confirm that TiPV is a novel parvovirus pathogen that can cause massive mortality in adult tilapia. This provides a basis for the further studies to define the epidemiology, pathology, diagnosis, prevention and treatment of this emerging viral disease. A novel parvovirus isolated from adult tilapia causes substantial morbidity and mortality. Using a SISPA-PCR and RACE, we identified and characterized 4269 nucleotides of this parvovirus. Tentatively named Tilapia parvovirus (TiPV), this is to our knowledge the first putative member of the family Parvoviridae shown to infect a teleost host. We found that a nucleotide sequence similarity search by BLASTX had no significant matches with other viruses, while amino acid sequence comparison indicated approximately 34.6% ~ 50.0% amino acids (aa) homology with other parvoviruses. Similarities between the genomes of parvoviruses infecting hosts in different phyla or divisions indicate a need to update previously suggested hypotheses on the origins of parvovirus. Our findings may represent new avenues to explain viral evolution and suggest a need to further study parvovirus pathogenesis.
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