Characterization of sperm-associated antigen 6 expression in the reproductive tract of the domestic rooster (Gallus domesticus) and its impact on sperm mobility.
Characterization of sperm-associated antigen 6 expression in the reproductive tract of the domestic rooster (Gallus domesticus) and its impact on sperm mobility.
复制标题
DOI：
10.1016/j.psj.2020.08.009
复制
发表时间：
2020-11
期刊：
影响因子：
4.4
通讯作者：
Benson AP
中科院分区：
文献类型：
--
作者：
Jarrell ZR;Ahammad MU;Sweeney K;Wilson JL;Benson AP
精子活动性是决定家鸡精子质量的主要因素，因此是提高生育力的一个重要方面。精子相关抗原6（SPAG 6）是一种重要的鞭毛蛋白，对鞭毛的功能是必需的，但与公鸡的生育力呈负相关。本研究的目的是表征SPAG6的表达，并探讨其作为精子运动的蛋白质生物标志物的实用性。通过Western分析，比较了睾丸、附睾和输精管组织以及顺序成熟精子中SPAG6的相对丰度。应用免疫细胞化学技术检测SPAG 6在鸡精子中的定位。最后，Western分析用于比较不同活动度精子中SPAG6的相对丰度。相对于睾丸，SPAG6在附睾组织中的丰度更高，在脉管组织中的丰度最高。还发现SPAG6在成熟精子中的丰度依次增加。SPAG6定位于精子鞭毛中的轴丝中心微管对之间，但在顶体区域中也发现了较低浓度的SPAG6。SPAG 6不是精子运动性的重要预测因素。SPAG6丰度本身并不是精子活动性的强有力预测因素。它对公鸡生育力的影响可能与其对精子活动性的影响无关。
Sperm mobility is a major determinant of sperm quality in the domesticated chicken (Gallus domesticus) and is therefore an area of interest for improving fertility. Sperm-associated antigen 6 (SPAG6) is an important flagellar protein implicated to be necessary for flagellar function but negatively associated with rooster fertility. This study was aimed to characterize the expression of SPAG6 and investigate its utility as a protein biomarker of sperm mobility. By western analysis, relative SPAG6 abundances were compared between the testicular, epididymal, and vasal tissues and in sequentially maturing sperm. Immunocytochemistry techniques were used to detect localization of SPAG6 in chicken sperm. Last, western analysis was used to compare relative SPAG6 abundances in sperm of differing mobility. SPAG6 was found in higher abundance in epididymal tissues and in highest abundance in vasal tissues, relative to that of the testis. SPAG6 was also found to sequentially increase in abundance in maturing sperm. SPAG6 localizes between the axonemal central pair of microtubules in the sperm flagella, but it is also found in lower concentration in the acrosomal region. SPAG6 was not a significant predictor of sperm mobility. SPAG6 abundance, alone, is not a strong predictor of sperm mobility. Its impact on rooster fertility is likely unrelated to its impact on sperm mobility.
登录
查看更多内容
影响因子：
3.6
作者：
Barbato, GF;Cramer, PG;Hammerstedt, RH
通讯作者：
Hammerstedt, RH
DOI：
10.1016/j.cbd.2016.10.006
发表时间：
2017-03-01
影响因子：
3
作者：
Atikuzzaman, Mohammad;Sanz, Libia;Rodriguez-Martinez, Heriberto
通讯作者：
Rodriguez-Martinez, Heriberto
影响因子：
2.3
作者：
Bi, Ye;Liu, Mingxi;Sha, Jiahao
通讯作者：
Sha, Jiahao
影响因子：
6.7
作者：
Kovac JR;Pastuszak AW;Lamb DJ
通讯作者：
Lamb DJ
影响因子：
4.4
作者：
BURROWS, W. H.;QUINN, J. P.
通讯作者：
QUINN, J. P.