Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.
Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.
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DOI：
10.2141/jpsa.2023017
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发表时间：
2023
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影响因子：
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血浆中Nτ-甲基组氨酸的浓度提供了骨骼肌蛋白质分解的指标。建立了鸡血浆中Nτ-甲基组氨酸及其异构体Nπ-甲基组氨酸的液相色谱-串联质谱（LC-MS/MS）定量分析方法。Nτ-甲基组氨酸和Nπ-甲基组氨酸的线性范围分别为1.56-50.00 μmol/L和0.78-25.00 μmol/L。拟定方法检测了禁食和再进食后Nτ-甲基组氨酸和Nπ-甲基组氨酸血浆水平的变化。结果表明，该方法可同时测定鸡血浆中Nτ-甲基组氨酸和Nπ-甲基组氨酸的浓度。
The concentration of Nτ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of Nτ-methylhistidine and its isomer Nπ-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for Nτ-methylhistidine and 0.78–25.00 μmol/L for Nπ-methylhistidine. The proposed method detected changes in the plasma levels of Nτ-methylhistidine and Nπ-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma.
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影响因子：
--
作者：
Huang J;Mondul AM;Weinstein SJ;Karoly ED;Sampson JN;Albanes D
通讯作者：
Albanes D
影响因子：
4.5
作者：
FORSBERG, NE;LIU, CC
通讯作者：
LIU, CC
DOI：
10.1016/0300-9629(95)00022-y
发表时间：
1995-07-01
影响因子：
2.3
作者：
SARTORI, DRS;MIGLIORINI, RH;LINDER, C
通讯作者：
LINDER, C
DOI：
10.1007/bf01145413
发表时间：
1976-01-01
期刊：
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG
影响因子：
--
作者：
TINBERGEN, BJ;SLUMP, P
通讯作者：
SLUMP, P
DOI：
10.2141/jpsa.0200030
发表时间：
2021-04-25
期刊：
The journal of poultry science
影响因子：
--
作者：
Makino R;Uda M;Shuto S;Kita K;Tachibana T
通讯作者：
Tachibana T