Seroprevalence of Helicobacter pylori infection in cystic fibrosis and its cross-reactivity with anti-pseudomonas antibodies.
Seroprevalence of Helicobacter pylori infection in cystic fibrosis and its cross-reactivity with anti-pseudomonas antibodies.
复制标题
囊性纤维化中幽门螺杆菌感染的血清阳性率及其与抗假单胞菌抗体的交叉反应性。
DOI：
10.1097/00005176-200004000-00015
复制
发表时间：
2000
影响因子：
2.9
通讯作者：
Gold,BD
中科院分区：
文献类型：
--
作者：
Israel,NR;Khanna,B;Cutler,A;Perry,M;Caplan,D;Weatherly,M;Gold,BD
背景：幽门螺杆菌感染的患病率及其在囊性纤维化（CF）胃十二指肠疾病中的作用存在争议。此外，由于与其他细菌物种的交叉反应性，该人群中感染的血清学测定可能不准确。H.方法：研究酶联免疫吸附测定（ELISA）和三种商业血清学测定（PyloriStat; BioWhittaker，Walkersville，MD，USA; Flexsure; SmithKlineDiagnostics，Inc.，圣何塞，CA，USA;和HM-CAP; EPI，斯托尼布鲁克，NY，USA）在三个独立实验室测定抗H.检测70例CF患者的pylori IgG抗体。固相H. pylori抗原和假单胞菌抗体通过竞争抑制试验确定，用来自不同假单胞菌属的全细胞蛋白预吸附CF患者血清，并通过每种试验进行血清再分析。结果：ELISA、Flexsure、Pyloristat、HM-CAP等方法初步检测到H。pylori血清阳性率分别为47%、28%、24%和37%。吸附后血清阳性率分别降至8%、0%、0%和15%。所有研究ELISA真阳性结果的患者经内镜证实为H。幽门感染Westernblot分析表明，在31 kDa的H. pylori蛋白与两种细菌共有的抗原表位。pylori抗原和抗假单胞菌抗体在CF患者中发生。在评价血清H. pylori测试在这个人群中。在血清学检测中，应使用假单胞菌蛋白对CF血清进行预吸附。
Background:The prevalence of Helicobacter pylori infection and its role in gastroduodenal disease in cystic fibrosis (CF) are controversial. Additionally, serologic determination of infection in this population may be inaccurate because of cross-reactivity with other bacterial species. The seroprevalence of H. pylori in a cohort of patients with CF and its cross-reactivity with Pseudomonas antibodies were investigated.Methods:A research enzyme-linked immunosorbent assay (ELISA), and three commercial serologic assays (PyloriStat; BioWhittaker, Walkersville, MD, USA; Flexsure; SmithKline Diagnostics, Inc., San Jose, CA, USA; and HM-CAP; EPI, Stony Brook, NY, USA) at three independent laboratories determined the seroprevalence of anti-H. pylori IgG antibodies in 70 patients with CF. Cross-reactivity between solid-phase H. pylori antigens and Pseudomonas antibodies was ascertained by a competitive inhibition assay, preadsorbing sera of patients with CF with whole cell proteins from different Pseudomonas species, and serum reanalysis by each assay. Western blot analysis before and after adsorption was performed to identify potential cross-reactive antigens.Results:The research ELISA, Flexsure, Pyloristat, and HM-CAP initially showed H. pylori seropositivity of 47%, 28%, 24%, and 37%, respectively. Postadsorption seropositivity declined to 8%, 0%, 0%, and 15%, respectively. All patients with research ELISA true-positive results were confirmed endoscopically to have H. pylori infection. Western blot analysis showed a 31-kDa H. pylori protein with antigenic epitopes common to both bacterial species.Conclusions:Cross-reactivity between solid-phase H. pylori antigens and anti-Pseudomonas antibodies occurs in patients with CF. A high index of suspicion should be assumed in evaluating results of serologic H. pylori tests in this population. Preadsorption of CF sera with Pseudomonas proteins should be used in serologic testing.
登录
查看更多内容
不同人群幽门弯曲菌感染的血清流行病学
影响因子：
9.4
作者：
F. Mégraud;M.;F. Denis;A. Belbouri;D. Q. Hoa
通讯作者：
D. Q. Hoa
囊性纤维化中铜绿假单胞菌抗原免疫反应的纵向研究
影响因子：
3.1
作者：
G. Döring;N. Høiby
通讯作者：
N. Høiby
幽门弯曲杆菌外膜蛋白的鉴定以及幽门弯曲杆菌和空肠弯曲杆菌之间的抗原交叉反应性。
DOI：
10.1099/00221287-133-1-163
发表时间：
1987
期刊：
Journal of general microbiology
影响因子：
--
作者：
D. Newell
通讯作者：
D. Newell
影响因子：
9.8
作者：
Marchildon, P;Balaban, DH;Peura, DA
通讯作者：
Peura, DA
肠杆菌科、铜绿假单胞菌和霍乱弧菌中的脂多糖结构与弯曲杆菌属具有免疫相关性
影响因子：
3.1
作者：
G. Perez;J. Hopkins;M. Blaser
通讯作者：
M. Blaser