Microbial community functional change during vertebrate carrion decomposition.
Microbial community functional change during vertebrate carrion decomposition.
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DOI：
10.1371/journal.pone.0079035
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Benbow ME
中科院分区：
文献类型：
--
作者：
Pechal JL;Crippen TL;Tarone AM;Lewis AJ;Tomberlin JK;Benbow ME
微生物在有机物的分解中发挥着关键作用，有机物有助于每个生态系统的能量和养分转化。然而，人们对与腐肉相关的坏死微生物群落的功能活动知之甚少。本研究的目的是利用不同季节、年份之间以及微生物群落与真核定殖者（例如食尸昆虫）分离时整个分解过程中碳源使用的差异来描述与腐肉相关的微生物群落功能活动。此外，在一项研究中使用高通量测序在系统水平上鉴定了微生物群落。我们假设腐肉微生物群落的功能特征会随着分解的持续时间而变化，并且这种变化将取决于季节、年份和食尸昆虫定殖的存在。 Biolog EcoPlates™ 用于通过脊椎动物腐肉分解过程中 29 种碳源的差异使用来测量坏死微生物群落功能的变化。焦磷酸测序用于描述一项实验中的细菌群落组成，以识别与群落功能变化相关的关键门。总体而言，微生物功能活性在春季、夏季和冬季的分解过程中增加，而在秋季则降低。此外，2011 年测试食尸节肢动物殖民者效应时，微生物功能活性更高。 2010年至2011年间，从尸食性昆虫聚居的遗骸中分离出来的群落的微生物功能存在不一致的趋势，这表明更需要对这一过程进行机械理解。这些数据表明功能分析可以在腐肉研究中实施，并且对于理解微生物群落对重要的生态系统过程（腐肉分解）的影响非常重要。
Microorganisms play a critical role in the decomposition of organic matter, which contributes to energy and nutrient transformation in every ecosystem. Yet, little is known about the functional activity of epinecrotic microbial communities associated with carrion. The objective of this study was to provide a description of the carrion associated microbial community functional activity using differential carbon source use throughout decomposition over seasons, between years and when microbial communities were isolated from eukaryotic colonizers (e.g., necrophagous insects). Additionally, microbial communities were identified at the phyletic level using high throughput sequencing during a single study. We hypothesized that carrion microbial community functional profiles would change over the duration of decomposition, and that this change would depend on season, year and presence of necrophagous insect colonization. Biolog EcoPlates™ were used to measure the variation in epinecrotic microbial community function by the differential use of 29 carbon sources throughout vertebrate carrion decomposition. Pyrosequencing was used to describe the bacterial community composition in one experiment to identify key phyla associated with community functional changes. Overall, microbial functional activity increased throughout decomposition in spring, summer and winter while it decreased in autumn. Additionally, microbial functional activity was higher in 2011 when necrophagous arthropod colonizer effects were tested. There were inconsistent trends in the microbial function of communities isolated from remains colonized by necrophagous insects between 2010 and 2011, suggesting a greater need for a mechanistic understanding of the process. These data indicate that functional analyses can be implemented in carrion studies and will be important in understanding the influence of microbial communities on an essential ecosystem process, carrion decomposition.
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影响因子：
3.7
作者：
Barton, Philip S.;Cunningham, Saul A.;Manning, Adrian D.
通讯作者：
Manning, Adrian D.
影响因子：
2.7
作者：
Barton, Philip S.;Cunningham, Saul A.;Manning, Adrian D.
通讯作者：
Manning, Adrian D.
影响因子：
4.4
作者：
GARLAND, JL;MILLS, AL
通讯作者：
MILLS, AL
影响因子：
4.8
作者：
Bucher, AE;Lanyon, LE
通讯作者：
Lanyon, LE
影响因子：
4.2
作者：
Dowd, Scot E.;Sun, Yan;Secor, Patrick R.;Rhoads, Daniel D.;Wolcott, Benjamin M.;James, Garth A.;Wolcott, Randall D.
通讯作者：
Wolcott, Randall D.