Pulmonary Microbiome of Patients Receiving Mechanical Ventilation: Changes Over Time.
Pulmonary Microbiome of Patients Receiving Mechanical Ventilation: Changes Over Time.
复制标题
接受机械通气患者的肺部微生物组：随时间的变化
DOI：
10.4037/ajcc2021194
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发表时间：
2021-03-01
期刊：
影响因子：
--
通讯作者：
Mehta D
中科院分区：
文献类型：
--
作者：
Sole ML;Yooseph S;Talbert S;Abomoelak B;Deb C;Rathbun KP;Penoyer D;Middleton A;Mehta D
对肺部微生物组的兴趣正在增长，特别是在接受机械通气的患者中。探索长期机械通气患者随时间推移的肺部微生物组，并评价口腔吸痰干预对微生物组的影响。这项来自临床试验的描述性亚组分析涉及16名接受机械通气至少5天的参与者（7名干预，9名对照）的随机样本。随着时间的推移，对每名参与者的5对口腔和气管标本进行评价。使用16 S rRNA基因测序评价配对标本的细菌DNA。计算细菌分类组成、α多样性（Shannon指数）和β多样性（Morisita-Horn指数），并在参与者内部和参与者之间进行比较。参与者主要为男性（69%）和白色（63%），平均年龄为58岁，平均接受机械通气9.36天。丰富的细菌类群包括普雷沃氏菌属、葡萄球菌属、链球菌属、寡养单胞菌属和韦荣氏菌属。总组（P = .002）和对照组（P = .02）的平均气管α多样性随时间推移而降低。对照组的β多样性（1.905）低于干预组（2.607）（P = 0.04）。长期机械通气与肺部微生物组的变化相关，对照组的多样性较低。口腔吸痰干预可能减少了口腔-气管细菌传播。
Interest in the pulmonary microbiome is growing, particularly in patients undergoing mechanical ventilation. To explore the pulmonary microbiome over time in patients undergoing prolonged mechanical ventilation and to evaluate the effect of an oral suctioning intervention on the microbiome. This descriptive subanalysis from a clinical trial involved a random sample of 16 participants (7 intervention, 9 control) who received mechanical ventilation for at least 5 days. Five paired oral and tracheal specimens were evaluated for each participant over time. Bacterial DNA from the paired specimens was evaluated using 16S rRNA gene sequencing. Bacterial taxonomy composition, α-diversity (Shannon index), and β-diversity (Morisita-Horn index) were calculated and compared within and between participants. Participants were predominantly male (69%) and White (63%), with a mean age of 58 years, and underwent mechanical ventilation for a mean of 9.36 days. Abundant bacterial taxa included Prevotella, Staphylococcus, Streptococcus, Stenotrophomonas, and Veillonella. Mean tracheal α-diversity decreased over time for the total group (P = .002) and the control group (P = .02). β-Diversity was lower (P = .04) in the control group (1.905) than in the intervention group (2.607). Prolonged mechanical ventilation was associated with changes in the pulmonary microbiome, with the control group having less diversity. The oral suctioning intervention may have reduced oral-tracheal bacterial transmission.
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影响因子：
18.2
作者：
Dickson RP;Erb-Downward JR;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
DOI：
10.1164/rccm.201210-1913oc
发表时间：
2013-05-15
影响因子：
24.7
作者：
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影响因子：
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作者：
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影响因子：
15.5
作者：
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通讯作者：
Collman RG
影响因子：
3.7
作者：
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通讯作者：
Allen MS