Full-length transcriptome profiling reveals insight into the cold response of two kiwifruit genotypes (A. arguta) with contrasting freezing tolerances.
Full-length transcriptome profiling reveals insight into the cold response of two kiwifruit genotypes (A. arguta) with contrasting freezing tolerances.
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全长转录组分析揭示了两种具有对比冷冻耐受性的猕猴桃基因型（A. arguta）的冷反应
DOI：
10.1186/s12870-021-03152-w
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发表时间：
2021-08-11
影响因子：
5.3
通讯作者：
Fang J
中科院分区：
文献类型：
--
作者：
Sun S;Lin M;Qi X;Chen J;Gu H;Zhong Y;Sun L;Muhammad A;Bai D;Hu C;Fang J
研究背景猕猴桃（ActinidiaLindl.）被认为是世界上重要的水果品种。由于其温带起源，该物种是非常容易受到冻害，而在低温胁迫。为了进一步了解抗冻性的机制，我们对两个A. Actinidiacutta（Actinidiacutta）基因型KL和RB，分别为高、低抗冻性基因型。两种基因型均在− 25 °C下放置0 h、1 h和4 h。结果使用从头方法组装SMRT（单分子实时）RNA-seq数据，产生24，306个unigenes，N50值为1834 bp。京都基因和基因组百科全书（KEGG）富集分析表明，DEG参与“淀粉和蔗糖代谢”、“丝裂原活化蛋白激酶（MAPK）信号通路”、“磷脂酰肌醇信号系统”、“肌醇磷酸代谢”和“植物激素信号转导”。特别是，对于“淀粉和蔗糖代谢”，我们确定了3个关键基因参与纤维素降解，海藻糖合成和淀粉降解过程。此外，低温胁迫还增强了上述3个关键基因所编码的β-葡萄糖苷酶（beta-GC）、海藻糖-6-磷酸合成酶（TPS）和β-淀粉酶（BAM）的活性。属于AP 2/ERF、bHLH（basic helix-loop-helix）和MYB家族的三种转录因子（TF）参与低温响应。此外，加权基因共表达网络分析（WGCNA）表明，β-GC、TPS 5和BAM 3. 1是影响猕猴桃低温反应的关键基因，与CBF 3、MYC 2和MYB 44基因高度共表达。低温显著影响淀粉和蔗糖代谢过程。淀粉和蔗糖代谢可能是耐低温猕猴桃抵御低温伤害的重要途径。这些结果增加了我们对低温胁迫下猕猴桃抗冻性复杂机制的理解，并揭示了一系列用于培育具有增强抗冻性的新品种的候选基因。
BackgroundKiwifruit (ActinidiaLindl.) is considered an important fruit species worldwide. Due to its temperate origin, this species is highly vulnerable to freezing injury while under low-temperature stress. To obtain further knowledge of the mechanism underlying freezing tolerance, we carried out a hybrid transcriptome analysis of twoA. arguta(Actinidi arguta) genotypes, KL and RB, whose freezing tolerance is high and low, respectively. Both genotypes were subjected to − 25 °C for 0 h, 1 h, and 4 h.ResultsSMRT (single-molecule real-time) RNA-seq data were assembled using the de novo method, producing 24,306 unigenes with an N50 value of 1834 bp. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs showed that they were involved in the ‘starch and sucrose metabolism’, the ‘mitogen-activated protein kinase (MAPK) signaling pathway’, the ‘phosphatidylinositol signaling system’, the ‘inositol phosphate metabolism’, and the ‘plant hormone signal transduction’. In particular, for ‘starch and sucrose metabolism’, we identified 3 key genes involved in cellulose degradation, trehalose synthesis, and starch degradation processes. Moreover, the activities of beta-GC (beta-glucosidase), TPS (trehalose-6-phosphate synthase), and BAM (beta-amylase), encoded by the abovementioned 3 key genes, were enhanced by cold stress. Three transcription factors (TFs) belonging to the AP2/ERF, bHLH (basic helix-loop-helix), and MYB families were involved in the low-temperature response. Furthermore, weighted gene coexpression network analysis (WGCNA) indicated thatbeta-GC,TPS5, andBAM3.1were the key genes involved in the cold response and were highly coexpressed together with theCBF3,MYC2, andMYB44genes.ConclusionsCold stress led various changes in kiwifruit, the ‘phosphatidylinositol signaling system’, ‘inositol phosphate metabolism’, ‘MAPK signaling pathway’, ‘plant hormone signal transduction’, and ‘starch and sucrose metabolism’ processes were significantly affected by low temperature. Moreover, starch and sucrose metabolism may be the key pathway for tolerant kiwifruit to resist low temperature damages. These results increase our understanding of the complex mechanisms involved in the freezing tolerance of kiwifruit under cold stress and reveal a series of candidate genes for use in breeding new cultivars with enhanced freezing tolerance.
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影响因子：
5.3
作者：
Feng XM;Zhao Q;Zhao LL;Qiao Y;Xie XB;Li HF;Yao YX;You CX;Hao YJ
通讯作者：
Hao YJ
影响因子：
5.6
作者：
Ma X;Xia H;Liu Y;Wei H;Zheng X;Song C;Chen L;Liu H;Luo L
通讯作者：
Luo L
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影响因子：
11.8
作者：
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6-磷酸海藻糖合酶基因 OsTPS1 的过度表达增强了水稻的非生物胁迫耐受性
影响因子：
4.3
作者：
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通讯作者：
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影响因子：
4.4
作者：
Dong L;Liu H;Zhang J;Yang S;Kong G;Chu JS;Chen N;Wang D
通讯作者：
Wang D